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Quercetin inhalation inhibits the asthmatic responses by exposure to aerosolized-ovalbumin in conscious guinea-pigs

  • Hee Moon
  • Hyun Ho Choi
  • Ji Yun Lee
  • Hyo Jin Moon
  • Sang Soo Sim
  • Chang Jong Kim
Articles Drug Efficacy and Safety

Abstract

Effects of quercetin inhalation on immediate (IAR), late-phase (LAR) and late late-phase (LLAR) asthmatic responses by exposure to aerosolized-ovalbumin (AOA) (2w/v% in saline, inhalation for 3 min) were studied in conscious guinea-pigs sensitized with AOA. We measured specific airway resistance (sRaw), and recruitment of leukocytes, histamine and protein contents and phospholipase A2 (PLA2) activity in bronchoalveolar lavage fluid (BALF). Effects of quercetin (10 mg/mL, inhalation for 2 min) compared with cromolyn sodium, salbutamol, and dexamethasone inhalations, respectively. Quercetin inhalation decreased sRaw by 57.15 ± 3.82% in IAR, 57.72 ± 7.28% in LAR, and 55.20 ± 5.69% in LLAR compared with AOA-inhaled control. Salbutamol inhalation (5 mg/mL) significantly inhibited sRaw in IAR, but inhalations of cromolyn sodium (10 mg/mL) and dexamethasone (5 mg/mL) significantly inhibited sRaw in IAR, LAR and LLAR, respectively. Inhibitory activity of quercetin inhalation on sRaw was similar to effect of its oral administration (10 mg/kg) in asthmatic responses. Quercetin (10 mg/mL, inhalation for 2 min) significantly decreased histamine and protein contents, PLA2 activity, and recruitments of leukocytes in BALF and also improved slightly infiltration of eosinophils and neutrophils in histopathological survey. Its anti-asthmatic activity was similar to cromolyn sodium and dexamethasone.

Key words

Quercetin inhalation Anti-asthmatic action Specific airway resistance Recruitment of eosinophil Bronchoalveolar lavage fluid Guinea pigs 

Abbreviations

AOA

Aerosolized ovalbumin

BALF

Brochoalveolar lavage fluid

BSA

Bovine serum albumin

BSS

Balanced salt solution

DMSO

Dimethylsulfoxide

EOS

Eosinophils

H & E

Hematoxylin and eosin

HEPES

4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid

IAR

Immediate-phase asthmatic response

IgE

Immunoglobulin E

LAR

Late-phase asthmatic response

LLAR

Late late-phase asthmatic response

OA

Ovalbumin

PLA2

Phospholipase A2

PMN

Polymorphonuclear leukocytes

10-Pyrene PC

Pyrene-labeled phospholipids

sRaw

Specific airway resistance

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Copyright information

© The Pharmaceutical Society of Korea 2008

Authors and Affiliations

  • Hee Moon
    • 1
  • Hyun Ho Choi
    • 1
  • Ji Yun Lee
    • 1
  • Hyo Jin Moon
    • 1
  • Sang Soo Sim
    • 1
  • Chang Jong Kim
    • 1
  1. 1.Division of Pathophysiology and Pharmacology, College of PharmacyChung-Ang UniversitySeoulKorea

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