Quercetin in a lotus leaves extract may be responsible for antibacterial activity
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In the course of a search for chemotherapeutic agents inhibiting suspected peridontitis bacteria, extracted and purified substances from lotus leaf were identified by antimicrobial activity tests with use of the broth micro-dilution methods on 96-microwell plate. The antimicrobial activity of extracts was tested against five microorganisms, namely: Actinobacillus actinomycetemcomitans Y4, Actinomyces viscosus 19246, Porphyromonas gingivalis 33277, Fusobacterium nucleatum 25586, and Actinomyces naeslundii wvl 45. The most active antimicrobial extract was subjected to spectroscopic analysis using UV, mass spectrometry, and by 1H, 13C-, nuclear magnetic resonance spectroscopy. Our data showed that the minimum inhibitory concentrations of the most active extract were 0.625, 1.25, 1.25, 0.625 and 2.5 mg/mL for A. actinomycetemcomitans, A. viscosus, P. gingivalis, F. nucleatum, and A. naeslundii, respectively. The component that had a greatest antimicrobial activity was determined to be quercetin. Thus, we conclude that quercetin extracted from lotus leaves may be a potential antibacterial agent for periodontitis.
Key wordsLotus leaf extracts Periodontitis bacteria Spectroscopic analysis
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- Cui, D. S., An approach of lotus leaf in Pediatrics. Chinese Traditional Medicine of SiChuan (Chinese), 14, 23–26 (1996).Google Scholar
- Kashiwada, Y., Aoshima, A., Ikeshiro, Y., Chen, Y.-P., Furukawa, H., Itoigawa, M., Fujioka, T., Mihashi, K., Cosentino, L. M., Morris-Natschke, S. L., and Lee, K.-H., Anti-HIV benzylisoquinoline alkaloids and flavonoids from the leaves of Nelumbo nucifera, and structure-activity correlations with related alkaloids. Bioorganic & Medicinal Chemistry., 13, 443–448 (2005).CrossRefGoogle Scholar
- Li, J. H., Research on Health Care Gum of Lotus Leaf and Honeysuckle, Food Science (Chinese)., 23, 23–27 (2004).Google Scholar
- Li, X. G. and He, X. D., Reduce heat drug. In: ZeiQuan Lei and TingMo Zhang (Eds.), Chinese clinical traditional medicine, 1st edition, People health press, Beijing, pp 431–432 (1998).Google Scholar
- National Committee for Clinical Laboratory Standards., Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically-Second Edition, Approved Standard M7-A2. NCCLS, Villanova, PA, USA, (1990).Google Scholar
- Tian, N., Liu, Z., Huang, J., Luo, G., Liu, S., and Liu, X., Isolation and preparation of flavonoids from the leaves of Nelumbo nucifera Gaertn by preparative reversed-phase high-performance liquid chromatography. Sepu., 25, 88–92 (2007).Google Scholar
- Xu, Z. and Li, M., Comparison of Inhibitions to Oral Bacteria among 10 Chinese Herbs. Chinese Journal of Modern Applied Pharmacy, 17, 282–284 (2000).Google Scholar
- Wassel, G., Saeed, A., Ibrahim, N., and El-Eraqy, W., Flavonoids of Nelumbo nucifera Gaertn and biological evaluation. Egyptian Journal of Pharmaceutical Sciences, 37, 585–596 (1996).Google Scholar
- Wu, A. J., Shao, J. J., and Zheng, L., Study of Magnolol on the treatment of dental caries, Stomatology (Chinese), 5, 115–117 (1993).Google Scholar