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Archives of Pharmacal Research

, Volume 31, Issue 5, pp 640–644 | Cite as

Quercetin in a lotus leaves extract may be responsible for antibacterial activity

  • Mingyu Li
  • Zhuting Xu
Articles Drug Efficacy and Safety

Abstract

In the course of a search for chemotherapeutic agents inhibiting suspected peridontitis bacteria, extracted and purified substances from lotus leaf were identified by antimicrobial activity tests with use of the broth micro-dilution methods on 96-microwell plate. The antimicrobial activity of extracts was tested against five microorganisms, namely: Actinobacillus actinomycetemcomitans Y4, Actinomyces viscosus 19246, Porphyromonas gingivalis 33277, Fusobacterium nucleatum 25586, and Actinomyces naeslundii wvl 45. The most active antimicrobial extract was subjected to spectroscopic analysis using UV, mass spectrometry, and by 1H, 13C-, nuclear magnetic resonance spectroscopy. Our data showed that the minimum inhibitory concentrations of the most active extract were 0.625, 1.25, 1.25, 0.625 and 2.5 mg/mL for A. actinomycetemcomitans, A. viscosus, P. gingivalis, F. nucleatum, and A. naeslundii, respectively. The component that had a greatest antimicrobial activity was determined to be quercetin. Thus, we conclude that quercetin extracted from lotus leaves may be a potential antibacterial agent for periodontitis.

Key words

Lotus leaf extracts Periodontitis bacteria Spectroscopic analysis 

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References

  1. Bathori, M., Zupko, I., and Hunyadi, A., Monitoring the antioxidant activity of extracts originated from various Serratula species and isolation of flavonoids from Serratula coronata. Fitoterapia, 75, 162–167 (2004).PubMedCrossRefGoogle Scholar
  2. Cui, D. S., An approach of lotus leaf in Pediatrics. Chinese Traditional Medicine of SiChuan (Chinese), 14, 23–26 (1996).Google Scholar
  3. Kanadaswami, C., Lee, L. T., and Lee, P. P., The antitumor activities of flavonoids. In Vivo, 19, 895–909 (2005).PubMedGoogle Scholar
  4. Kashiwada, Y., Aoshima, A., Ikeshiro, Y., Chen, Y.-P., Furukawa, H., Itoigawa, M., Fujioka, T., Mihashi, K., Cosentino, L. M., Morris-Natschke, S. L., and Lee, K.-H., Anti-HIV benzylisoquinoline alkaloids and flavonoids from the leaves of Nelumbo nucifera, and structure-activity correlations with related alkaloids. Bioorganic & Medicinal Chemistry., 13, 443–448 (2005).CrossRefGoogle Scholar
  5. Kubo, I., Muroi, H., and Himejiam, M., Antimicrobial activity of green tea flavor components and their combination effects. J. Agric. Food Chem., 40, 245–248 (1992).CrossRefGoogle Scholar
  6. Li, J. H., Research on Health Care Gum of Lotus Leaf and Honeysuckle, Food Science (Chinese)., 23, 23–27 (2004).Google Scholar
  7. Li, X. G. and He, X. D., Reduce heat drug. In: ZeiQuan Lei and TingMo Zhang (Eds.), Chinese clinical traditional medicine, 1st edition, People health press, Beijing, pp 431–432 (1998).Google Scholar
  8. Namba, T., Tsunezuka, M., and Hattori, H., Dental caries prevention by traditional Chinese medicines. Planta. Med., 44, 100–106 (1982).PubMedCrossRefGoogle Scholar
  9. National Committee for Clinical Laboratory Standards., Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically-Second Edition, Approved Standard M7-A2. NCCLS, Villanova, PA, USA, (1990).Google Scholar
  10. Ng, T. B., Liu, F., and Lu, Y., Antioxidant activity of compounds from the medicinal herb Aster tataricus. Comp. Biochem. Physiol. C Toxicol. Pharmacol., 136, 109–115 (2003).PubMedCrossRefGoogle Scholar
  11. Ooshima, T. and Minami, T., Reduction of dental plaque deposition in humans by Oolong tea extract. Caries Res., 28, 146–151 (1994).PubMedGoogle Scholar
  12. Rauha, J. P., Remes, S., and Heinonen, M., Antimicrobial effects of Finnish plant extracts containing flavonoids and other phenolic compounds. Int. J. Food Microbiol., 56, 3–12 (2000).PubMedCrossRefGoogle Scholar
  13. Tian, N., Liu, Z., Huang, J., Luo, G., Liu, S., and Liu, X., Isolation and preparation of flavonoids from the leaves of Nelumbo nucifera Gaertn by preparative reversed-phase high-performance liquid chromatography. Sepu., 25, 88–92 (2007).Google Scholar
  14. Xu, Z. and Li, M., Comparison of Inhibitions to Oral Bacteria among 10 Chinese Herbs. Chinese Journal of Modern Applied Pharmacy, 17, 282–284 (2000).Google Scholar
  15. Wassel, G., Saeed, A., Ibrahim, N., and El-Eraqy, W., Flavonoids of Nelumbo nucifera Gaertn and biological evaluation. Egyptian Journal of Pharmaceutical Sciences, 37, 585–596 (1996).Google Scholar
  16. Wennstrom, J. and Linhe, J., Some effects of a sanguinarine-containing mouthwash on developing plaque and gingivitis. J Clin Periodontol., 12, 86–93 (1985).CrossRefGoogle Scholar
  17. Wolinsky, L. E. and Sote, O. E., Isolation of natural plaque-inhibiting substances from “Nigerian Chewing Sticks”. Caries Res., 18, 216–225 (1984).PubMedCrossRefGoogle Scholar
  18. Wu, A. J., Shao, J. J., and Zheng, L., Study of Magnolol on the treatment of dental caries, Stomatology (Chinese), 5, 115–117 (1993).Google Scholar
  19. Wu-yuan, C. D., Green, L., and Brich, W. X., In vitro screening of Chinese medicinal toothpastes: Their effects on growth and plaque formation of Mutans Streptococci. Caries Res., 24, 198–202 (1990).PubMedCrossRefGoogle Scholar

Copyright information

© The Pharmaceutical Society of Korea 2008

Authors and Affiliations

  1. 1.Shanghai 8th hospital ShanghaiShanghaiChina
  2. 2.Shanghai Research Institute of StomatologyShanghai Jiao Tong UniversityShanghaiP.R.China

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