Skip to main content
SpringerLink
Log in

Mikrofluidische Einzelzelluntersuchungen mikrobieller Interaktionen

  • Mikrobiologie und Einzelzellanalyse
  • Published:
BIOspektrum Aims and scope

Abstract

Interspecies interactions inside microbial communities bear a tremendous diversity of complex chemical processes that are by far not understood. For the investigation of these interactions, precise analytical tools are often missing. This article presents microfluidic single-cell tools that can be used for dynamic growth analysis of contact-based (here: horizontal gene transfer) and contact free (diffusive) interactions (here: L-lysine exchange) between microbial cells and colonies on single-cell level.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literatur

  1. Braga RM, Dourado MN, Araújo WL (2016) Microbial interactions: ecology in a molecular perspective. Braz I Microbiol 47:86–98

    CAS  Google Scholar 

  2. Groβkopf T, Soyer OS (2014) Synthetic microbial communities. Curr Opin Microbiol 18:72–77

    Article  CAS  Google Scholar 

  3. Daims H, Taylor MW, Wagner M (2006) Wastewater treatment: a model system for microbial ecology. Trends Biotechnol 24:483–489

    CAS  PubMed  Google Scholar 

  4. Santhanam R, Luu VT, Weinhold A et al. (2015) Native root-associated bacteria rescue a plant from a sudden-wilt disease that emerged during continuous cropping. Proc Natl Acad Sci USA 112:E5013–E5020

    CAS  Google Scholar 

  5. Zhou K, Qiao K, Edgar S et al. (2015) Distributing a metabolic pathway among a microbial consortium enhances production of natural products. Nat Biotechnol 33:377–383

    CAS  PubMed  Google Scholar 

  6. Grünberger A, Probst C, Helfrich S et al. (2015) Spatiotemporal microbial single-cell analysis using a high-throughput microfluidics cultivation platform. Cytom Part A 87:1101–1115

    Google Scholar 

  7. Grünberger A, Wiechert W, Kohlheyer D (2014) Single-cell microfluidics: opportunity for bioprocess development. Curr Opin Biotechnol 29:15–23

    PubMed  Google Scholar 

  8. Burmeister A, Hilgers F, Langner A et al. (2019) A microfluidic co-cultivation platform to investigate microbial interactions at defined microenvironments. Lab Chip 19:98–110

    CAS  Google Scholar 

  9. Griffiths AIF, Gelbart WM, Miller IH et al. (1999) Modern Genetic Analysis. W. H. Freeman, New York

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Multiscale Bioengineering, Universität Bielefeld, Universitätsstraße 25, D-33615, Bielefeld, Deutschland

    Alina Burmeister &  Alexander Grunberger

  2. Institut Für Bio- Und Geowissenschaften, Ibg-1: Biotechnologie, Forschungszentrum Jülich, Jülich, Deutschland

    Alina Burmeister

  3. Institut Für Molekulare Enzymtechnologie, Universität Düsseldorf, Forschungszentrum Jülich, Jülich, Deutschland

    Fabienne Hilgers

Authors
  1. Alina Burmeister
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Fabienne Hilgers
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Alexander Grunberger
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Alexander Grunberger.

Additional information

Alina Burmeister 2010–2017 Biotechnologiestudium an der Hochschule für Angewandte Wissenschaften Hamburg. Seit 2017 Promotion an der Technischen Fakultät der Universität Bielefeld in der Arbeitsgruppe „Multiscale Bioengineering" von Jun.-Prof. Dr. A. Grünberger in Kooperation mit dem Forschungszentrum Jülich GmbH in der Arbeitsgruppe „Micro-scale Bioengineering” von Jun.-Prof. Dr. D. Kohlheyer.

Fabienne Hilgers 2011–2016 Biochemiestudium an der Universität Düsseldorf. Seit 2017 Promotion am Institut für Molekulare Enzymtechnologie (Leitung: Prof. Dr. K.-E. Jaeger) der Universität Düsseldorf im Forschungszentrum Jülich in der Arbeitsgruppe „Bakterielle Photo-biotechnologie" von Dr. T. Drepper.

Alexander Griinberger 2004–2010 Bioingenieurstudium an der Universitat Karlsruhe (TH). 2010–2014 Promotion am Institut fur Bio- und Geowissenschaften (IBG) des Forschungszentrums Jülich GmbH in der Arbeitsgruppe „Microscale Bioengineering" und der RWTH Aachen bei Prof. Dr. W. Wiechert. 2014–2017 Postdoktorand am IGB in der Arbeitsgruppe „Micro-scale Bioengineering". Seit 2017 W1-Professur fur Multiscale Bioengineering an der Technischen Fakultät der Universität Bielefeld und seit 2019 Mitglied im CeBiTec (The Center for Biotechnology) der Universität Bielefeld.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Burmeister, A., Hilgers, F. & Grunberger, A. Mikrofluidische Einzelzelluntersuchungen mikrobieller Interaktionen. Biospektrum 25, 515–519 (2019). https://doi.org/10.1007/s12268-019-1088-9

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12268-019-1088-9

Search

Navigation