Abstract
Glycosylation is an important post-translational modification that can be found in many eukaryotic proteins. Since it can be crucial for protein structure and function, properly glycosylated proteins are of paramount importance for crystal structure determination. The eukaryotic expression system LEXSY, based on the protozoan Leishmania tarentolae, has been used to express and crystallize fully glycosylated Arabidopsis thaliana legumain γ and elucidate the role of glycosylation for its function.
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Open Access: This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (https://doi.org/creativecommons.org/licenses/by/4.0/), which permits use, duplication, adaption, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
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Dall, E., Licht, A. & Brandstetter, H. Expression und Untersuchung von Pflanzenproteinen mit Leishmania tarentolae. Biospektrum 25, 60–61 (2019). https://doi.org/10.1007/s12268-019-0994-1
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DOI: https://doi.org/10.1007/s12268-019-0994-1