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BIOspektrum

, Volume 24, Issue 6, pp 616–617 | Cite as

Erfassung der Internalisierung von GPCRs mit CRISPR/Cas9

  • Andrea Krumm
  • Carl White
  • Kevin Pfleger
Anwendungen & Produkte G-Protein-gekoppelte Rezeptoren (GPCRs)
  • 10 Downloads

Abstract

Receptors are prevalent drug targets as they translate signals from outside the cell to cellular reactions. In drug finding and characterization it is not only required to measure ligand binding, but just as well subsequent regulatory steps including desensitizing protein interactions. Here, we show how CRISPR/Cas9 allowed endogenous expression of a G-protein coupled receptor (GPCR) and how that was used to study protein inter - actions and internalization of a cancer progressing receptor.

References

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    Sriram K, Insel PA (2018) GPCRs as targets for approved drugs: How many targets and how many drugs? Mol Pharmacol 93:251–258CrossRefPubMedGoogle Scholar
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    Sun X, Cheng G, Hao M et al. (2010) CXCL12/CXCR4/CXCR7 chemokine axis and cancer progression. Cancer Metastasis Rev 29:709–722CrossRefPubMedPubMedCentralGoogle Scholar
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    White CW, Vanyai HK, See HB et al. (2017) Using nanoBRET and CRISPR/Cas9 to monitor proximity to a genome-edited protein in real-time. Sci Rep 7:3187CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer-Verlag GmbH Deutschland, ein Teil von Springer Nature 2018

Authors and Affiliations

  1. 1.BMG LABTECHOrtenberg GmbH Allmendgrün 8OrtenbergDeutschland
  2. 2.Harry Perkins Institute of Medical ResearchPerthAustralia
  3. 3.Dimerix LimitedNedlandsAustralia

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