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BIOspektrum

, Volume 24, Issue 6, pp 601–603 | Cite as

PCR-basierte Verfahren für die Muta - tionsdetektion bei Tumorpatienten

  • Tamara V. Werner
  • Silke Laßmann
Wissenschaft · Special: PCR & qPCR Molekulare Diagnostik
  • 14 Downloads

Abstract

Liquid biopsy offers the potential for non-invasive monitoring and mutation detection in cancer. Detection of mutations with very low allele frequencies using PCR based technologies is a technical challenge. Several quantitative PCR based systems are integrated in routine molecular diagnostics. The detection limit may not always suffice with these qPCR systems, but may be achieved by new digital PCR systems.

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References

  1. [1]
    Diaz-Serrano A, Gella P, Jimenez E et al. (2018) Targeting EGFR in lung cancer: current standards and developments. Drugs, doi: 10.1007/s40265-018-0916-4Google Scholar
  2. [2]
    Siravegna G, Marsoni S, Siena S et al. (2017) Integrating liquid biopsies into the management of cancer. Nat Rev Clin Oncol 14:531–548CrossRefPubMedGoogle Scholar
  3. [3]
    Wan JCM, Massie C, Garcia-Corbacho J et al. (2017) Liquid biopsies come of age: towards implementation of circulating tumour DNA. Nat Rev Cancer 17:223–238CrossRefPubMedGoogle Scholar
  4. [4]
    Navarro E, Serrano-Heras G, Castano MJ et al. (2015) Realtime PCR detection chemistry. Clin Chim Acta 439:231–250CrossRefPubMedGoogle Scholar
  5. [5]
    Ye S, Dhillon S, Ke X et al. (2001) An efficient procedure for genotyping single nucleotide polymorphisms. Nucleic Acids Res 29:E88–8CrossRefPubMedPubMedCentralGoogle Scholar
  6. [6]
    Powell MJ, Ganta M, Peletskaya E et al. (2015) High Sensitivity Detection of Tumor Gene Mutations. BAOJ Cancer Sciences 1:001Google Scholar
  7. [7]
    Syed YY (2016) therascreen® EGFR RGQ PCR Kit: a companion diagnostic for afatinib and gefitinib in non-small cell lung cancer. Mol Diagn Ther 20:191–198CrossRefPubMedGoogle Scholar
  8. [8]
    Angulo B, Lopez-Rios F, Gonzalez D (2014) A new generation of companion diagnostics: cobas BRAF, KRAS and EGFR mutation detection tests. Expert Rev Mol Diagn 14:517–524CrossRefPubMedGoogle Scholar
  9. [9]
    Leitlinienprogramm Onkologie (Deutsche Krebsgesellschaft, Deutsche Krebshilfe, AWMF) (2018) Prävention, Diagnostik, Therapie und Nachsorge des Lungenkarzinoms, Langversion 1.0, AWMF-Registernummer: 020/007OL, http://leitlinienprogramm-onkologie.de/ Lungen karzinom.98.0.htmlGoogle Scholar
  10. [10]
    Vogelstein B, Kinzler KW (1999) Digital PCR. Proc Natl Acad Sci USA 96:9236–9241CrossRefPubMedGoogle Scholar
  11. [11]
    Hindson BJ, Ness KD, Masquelier DA et al. (2011) Highthroughput droplet digital PCR system for absolute quantitation of DNA copy number. Anal Chem 83:8604–8610CrossRefPubMedPubMedCentralGoogle Scholar
  12. [12]
    Diehl F, Li M, He Y et al. (2006) BEAMing: single-molecule PCR on microparticles in water-in-oil emulsions. Nat Methods 3:551–559CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag GmbH Deutschland, ein Teil von Springer Nature 2018

Authors and Affiliations

  1. 1.Institut für Klinische PathologieUniversitätsklinikum FreiburgFreiburg im BreisgauDeutschland
  2. 2.Deutsches Konsortium für Translationale Krebsforschung (Dktk), Partnerstandort FreiburgUnd Deutsches Krebsforschungszentrum (Dkfz)HeidelbergDeutschland

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