, Volume 23, Issue 6, pp 630–633 | Cite as

Baukasten der Natur: neue Proteine aus konservierten Fragmenten

  • Saacnicteh Toledo-Patino
  • Francisco Lobos
  • Birte HöckerEmail author
Wissenschaft Proteindesign


Proteins evolved to be very diverse and adapted to a multitude of central cellular functions. This impressive repertoire of today’s proteins developed through duplication and recombination of smaller protein and peptide building blocks. Based on such evolutionary observations we proposed a rational design strategy in which new functional hybrids can be build from fragments of existing proteins. With this approach we are tackling the long-standing goal of complex custom-made protein design.


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  1. [1]
    Söding J, Lupas AN (2003) More than the sum of their parts: on the evolution of proteins from peptides. Bioessays 25:837–846CrossRefPubMedGoogle Scholar
  2. [2]
    Söding J (2005) Protein homology detection by HMM-HMM comparison. Bioinformatics 21:951–960CrossRefPubMedGoogle Scholar
  3. [3]
    Farias-Rico JA, Schmidt S, Höcker B (2014) Evolutionary relationship of two ancient protein superfolds. Nat Chem Biol 10:710–715CrossRefPubMedGoogle Scholar
  4. [4]
    Höcker B, Schmidt S, Sterner R (2002) A common evolutionary origin of two elementary enzyme folds. FEBS Lett 510:133–135CrossRefPubMedGoogle Scholar
  5. [5]
    Höcker B (2014) Design of proteins from smaller fragments–learning from evolution. Curr Opin Struct Biol 27:56–62CrossRefPubMedGoogle Scholar
  6. [6]
    Bharat TA, Eisenbeis S, Zeth K et al. (2008) A beta alpha-barrel build by the combination of fragments from different folds. Proc Natl Acad Sci USA 105:9942–9947CrossRefPubMedPubMedCentralGoogle Scholar
  7. [7]
    Eisenbeis S, Proffitt W, Coles M et al. (2012) Potential of fragment recombination for rational design of proteins. J Am Chem Soc 134:4019–4022CrossRefPubMedGoogle Scholar
  8. [8]
    Shanmugaratnam S, Eisenbeis S, Höcker B (2012) A highly stable protein chimera built from fragments of different folds. Protein Eng Des Sel 25:699–703CrossRefPubMedGoogle Scholar
  9. [9]
    Alva V, Söding J, Lupas AN (2015) A vocabulary of ancient peptides at the origin of folded proteins. Elife 4:e09410CrossRefPubMedPubMedCentralGoogle Scholar
  10. [10]
    Farias-Rico JA, Höcker B (2013). Design of chimeric proteins by combination of subdomain-sized fragments. Methods Enzymol 523:389–405CrossRefGoogle Scholar
  11. [11]
    Huang PS, Feldmeier K, Parmeggiani F et al. (2016) De novo design of a fourfold symmetric TIM-barrel protein with atomic level accuracy. Nat Chem Biol 12:29–34CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag GmbH Deutschland 2017

Authors and Affiliations

  • Saacnicteh Toledo-Patino
    • 1
  • Francisco Lobos
    • 1
  • Birte Höcker
    • 1
    Email author
  1. 1.Lehrstuhl für BiochemieUniversität BayreuthBayreuthDeutschland

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