, Volume 23, Issue 3, pp 286–288 | Cite as

Maßgeschneiderte Methoden und Technologien für high content imaging

Wissenschaft · Special: High Content Imaging Methoden der Bildgebung


High content imaging has become an indispensable and probably the most frequently applied research tool in modern cell biology. To make use of its versatile applicability, the available instrumental infrastructure has to meet specific requirements which are defined by the employed experimental approach and increase along with the complexity of the assay technique used for assessing a specific biological question. Exemplary assay techniques and technology of various complexity are portrayed in this article.


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  1. [1]
    Spitzer P, Condic M, Herrmann M et al. (2016) Amyloidogenic amyloid-β-peptide variants induce microbial agglutination and exert antimicrobial activity. Sci Rep 6:32228CrossRefPubMedPubMedCentralGoogle Scholar
  2. [2]
    Gilbert DF, Meinhof T, Pepperkok R et al. (2009) DetecTiff: a novel image analysis routine for high-content screening microscopy. J Biomol Screen 14:944–955CrossRefPubMedGoogle Scholar
  3. [3]
    Walzik MP, Vollmar V, Lachnit T et al. (2015) A portable low-cost long-term live-cell imaging platform for biomedical research and education. Biosens Bioelectron 64:639–649CrossRefPubMedGoogle Scholar
  4. [4]
    Balansa W, Islam R, Gilbert DF et al. (2013) Australian marine sponge alkaloids as a new class of glycine-gated chloride channel receptor modulator. Bioorg Med Chem 21:4420–4425CrossRefPubMedGoogle Scholar
  5. [5]
    Gilbert DF, Islam R, Lynagh T et al. (2009) High throughput techniques for discovering new glycine receptor modulators and their binding sites. Front Mol Neurosci 2:17CrossRefPubMedPubMedCentralGoogle Scholar
  6. [6]
    Talwar S, Lynch JW, Gilbert DF (2013) Fluorescence-based high-throughput functional profiling of ligand-gated ion channels at the level of single cells. PloS One 8:e58479CrossRefPubMedPubMedCentralGoogle Scholar
  7. [7]
    Schneidereit D, Kraus L, Meier JC et al. (2016) Step-bystep guide to building an inexpensive 3D printed motorized positioning stage for automated high-content screening microscopy. Biosens Bioelectron 92:472–481CrossRefPubMedGoogle Scholar
  8. [8]
    Kuenzel K, Friedrich O, Gilbert DF (2016) A recombinant human pluripotent stem cell line stably expressing halide-sensitive YFP-I152L for GABAAR and GlyR-targeted high-throughput drug screening and toxicity testing. Front Mol Neurosci 9:51CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2017

Authors and Affiliations

  1. 1.Lehrstuhl für Medizinische BiotechnologieFriedrich-Alexander-Universität Erlangen-NürnbergErlangenDeutschland

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