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BIOspektrum

, Volume 23, Issue 1, pp 61–62 | Cite as

Chemisch definiert – ein zellbasierter Zytotoxizitätsassay ohne fötales Kälberserum

  • Joachim WiestEmail author
Anwendungen & Produkte Zellbiologische Methoden
  • 43 Downloads

Abstract

Tests for cytotoxicity–respectively biocompatibility–are performed e. g. for biological evaluation of medical devices or during the development of microphysiometric systems. Here, often cell culture methods which involve the use of fetal bovine serum (FBS) are employed. However, using FBS raises scientific and ethical concerns. In the presented work a protocol for a chemically defined test for cytotoxicity is described.

Literatur

  1. [1]
    van der Valk J, Brunner D, De Smet K et al. (2010) Optimization of chemically defined cell culture media–replacing fetal bovine serum in mammalian in vitro methods. Toxicol In Vitro 24:1053–1063CrossRefPubMedGoogle Scholar
  2. [2]
    van der Valk J et al. (2017) Fetal bovine serum (FBS): past–present–future. (Veröffentlichung in Vorbereitung)Google Scholar
  3. [3]
    Gstraunthaler G, Lindl T (2013) Zell-und Gewebekultur. Springer Spektrum, HeidelbergCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2017

Authors and Affiliations

  1. 1.cellasys GmbH - R&DMünchenDeutschland

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