, Volume 22, Issue 5, pp 532–534 | Cite as

Kombinatorische Chemie im hochdichten Arrayformat

Biochemische Synthese
  • Felix Löffler
  • Frank Breitling
  • Alexander Nesterov-Müller


We synthesize peptide arrays by using laser pulses to transfer amino acids onto a synthesis slide that are embedded in a polymer. After patterning these razor-thin spots, the coupling starts by heating the polymer that now serves as a solvent to couple monomers to the surface. The main advantages of this nanolayer solid phase chemistry are the high degree of automation, the very high spot density, and the in situ activation of monomers due to mixing different reagents in the stacked nanolayers.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. [1]
    Greenblatt CL, Davis A, Clement BG et al. (1999) Diversity of microorganisms isolated from amber. Microb Ecol 38:58–68CrossRefPubMedGoogle Scholar
  2. [2]
    Cano RJ, Borucki M (1995) Revival and identification of bacterial spores in 25- to 40-million-year-old Dominican amber. Science 268:1060–1064CrossRefPubMedGoogle Scholar
  3. [3]
    Beyer M, Nesterov A, Block I et al. (2007) Combinatorial synthesis of peptide arrays onto a microchip. Science 318:1888CrossRefPubMedGoogle Scholar
  4. [4]
    Stadler V, Felgenhauer T, Beyer M et al. (2008) Combinatorial synthesis of peptide arrays with a laser printer. Angew Chem Inter Ed 47:7132–7135CrossRefGoogle Scholar
  5. [5]
    Loeffler FF, Foertsch TC, Popov R et al. (2016) High-flexibility combinatorial peptide synthesis with laser-based transfer of monomers in solid matrix material. Nat Commun 7:11844CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Felix Löffler
    • 1
  • Frank Breitling
    • 1
  • Alexander Nesterov-Müller
    • 1
  1. 1.Institut für MikrostrukturtechnikKarlsruher Institut für Technologie (KIT)Eggenstein-LeopoldshafenDeutschland

Personalised recommendations