Neues Enzym mit Reverse Transkriptaseund DNA-Polymerase-Funktion
Anwendungen & Produkte Reverse Transkriptase-Polymerase-Kettenreaktion (RT-PCR)
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Abstract
Reverse transcriptions under high temperatures can be performed with a new designed, thermostable DNA polymerase. This DNA polymerase was generated from Taq DNA polymerase by directed evolution in multiple rounds of mutagenesis and screening. The new DNA polymerase combines an artificially induced reverse transcriptase activity with the natural thermostability of Taq DNA polymerase. Potential applications are depicted here.
Literatur
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© Springer-Verlag Berlin Heidelberg 2016