Eine Roboterplattform für das Hochdurchsatz-Screening von Biokatalysatoren
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We have established an automated robotic platform to perform all steps required for enzyme production and analysis in a microtiter plate format. This system covers colony picking, growth, protein expression, enzyme isolation and in-depth biochemical characterization using spectrophotometric and fluorimetric assays. It has successfully been applied to screen mutant libraries of biocatalysts for e.g., substrate scope, enantioselectivity or stability for various enzyme classes such as transaminases, Baeyer-Villiger monoxygenases, esterases and dehalogenases.