BIOspektrum

, Volume 21, Issue 2, pp 230–231 | Cite as

Eine Roboterplattform für das Hochdurchsatz-Screening von Biokatalysatoren

  • Mark Dörr
  • Dominique Böttcher
  • Anke Hummel
  • Matthias Höhne
  • Uwe Bornscheuer
Biotechnologie Enzymtechnologie

Abstract

We have established an automated robotic platform to perform all steps required for enzyme production and analysis in a microtiter plate format. This system covers colony picking, growth, protein expression, enzyme isolation and in-depth biochemical characterization using spectrophotometric and fluorimetric assays. It has successfully been applied to screen mutant libraries of biocatalysts for e.g., substrate scope, enantioselectivity or stability for various enzyme classes such as transaminases, Baeyer-Villiger monoxygenases, esterases and dehalogenases.

Literatur

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Mark Dörr
    • 1
  • Dominique Böttcher
    • 1
  • Anke Hummel
    • 1
  • Matthias Höhne
    • 1
  • Uwe Bornscheuer
    • 1
  1. 1.Institut für Biochemie, Abteilung Biotechnologie und EnzymkatalyseErnst-Moritz-Arndt-Universität GreifswaldGreifswaldDeutschland

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