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BIOspektrum

, Volume 20, Issue 3, pp 284–287 | Cite as

Identifizierung von Promotoren in Corynebacterium glutamicum

  • Tobias Busche
  • Katharina Pfeifer-Sancar
  • Christian Rückert
  • Jörn KalinowskiEmail author
Wissenschaft · Special: Genomics Transkriptom-Sequenzierung
  • 92 Downloads

Abstract

The high-throughput sequencing of cDNA derived from whole transcript — omes (RNA-Seq) has revolutionized studies of bacterial transcription. We optimized and applied RNA-Seq for the genome-wide determination of transcription starts and their associated promoters using as an example of the biotechnologically relevant microorganism Corynebacterium glutamicum. By applying a method named 5′-RNA-Seq we were able to identify thousands of promoters in this organism.

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Literatur

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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Tobias Busche
    • 1
  • Katharina Pfeifer-Sancar
    • 1
  • Christian Rückert
    • 1
  • Jörn Kalinowski
    • 1
    Email author
  1. 1.Mikrobielle Genomik und Biotechnologie, Centrum für Biotechnologie (CeBiTec)Universität BielefeldBielefeldGermany

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