Transkriptionsregulatoren im Dienste der Biotechnologie
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Microbes have evolved a variety of sensor devices for the intracellular detection of small molecules. This capability was recently harnessed for the construction of transcription factor-based biosensors translating the intracellular amino acid accumulation in Corynebacterium glutamicum into a fluorescent readout. These systems were successfully implemented in FACS high throughput screening approaches and allow the study of microbial populations at the single cell level.
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