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Einzelzell-Genomik: das Bakterium als Individuum

  • Wissenschaft · Methoden
  • Mikrobielle Ökologie
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Abstract

The concept of single-cell genomics lies in the recognition of each bacterial cell as a unique and distinct entity. Single-cell genomics employ phi29 polymerase and random hexamer primers to yield microgram-range genomic DNA from single microbial cells. The unprecedented insights obtained from single-cell genome analyses have allowed major advances in the fields of environmental microbiology, symbiosis, evolution, and bioprospecting.

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Literatur

  1. Nature Editorial (2011) Microbiology by numbers. Nat Rev Microbiol 9:628

    Article  Google Scholar 

  2. Ley RE, Turnbaugh PJ, Klein S et al. (2006) Human gut microbes linked to obesity. Nature 444:1022–1023

    Article  PubMed  CAS  Google Scholar 

  3. Bercik P, Denou E, Collins J et al. (2011) The intestinal microbiota affect central levels of brain-derived neurotropic factor and behavior in mice. Gastroenterology 141:599–609, 609.e1-3

    Article  PubMed  CAS  Google Scholar 

  4. Kamke J, Bayer K, Woyke T et al. (2012) Exploring symbioses by single-cell genomics. Biol Bull (im Druck)

  5. Hutchison CA, Venter JC (2006) Single-cell genomics. Nat Biotechnol 24:657–658

    Article  PubMed  CAS  Google Scholar 

  6. Lasken RS (2007) Single-cell genomic sequencing using multiple displacement amplification. Curr Opin Microbiol 10:510–516

    Article  PubMed  CAS  Google Scholar 

  7. Blainey PC, Quake SR (2011) Digital MDA for enumeration of total nucleic acid contamination. Nucleic Acids Res 39:e19

    Article  PubMed  Google Scholar 

  8. Woyke T, Xie G, Copeland A et al. (2009) Assembling the marine metagenome, one cell at a time. PLoS ONE 4:e5299

    Article  PubMed  Google Scholar 

  9. Siegl A, Kamke J, Hochmuth T et al. (2011) Single-cell genomics reveals the lifestyle of poribacteria, a candidate phylum symbiotically associated with marine sponges. ISME J 5:61–70

    Article  PubMed  Google Scholar 

  10. Siegl A, Hentschel U (2010) PKS and NRPS gene clusters from microbial symbiont cells of marine sponges by whole genome amplification. Environ Microbiol Rep 2:507–513

    Article  CAS  Google Scholar 

  11. Bayer K, Scheuermayer M, Fieseler L et al. (2012) Genomic mining for novel FADH2-dependent halogenases in marine sponge-associated microbial consortia. Mar Biotechnol. doi: 10.1007/s10126-012-9455-2

  12. Siegl A (2009) Einzelzell-basierte Methoden zur Charakterisierung Schwamm-assoziierter Bakterien. Dissertation, Universität Würzburg

  13. Hentschel U, Piel J, Degnan SM et al. (2012) Genomic insights into the marine sponge microbiome. Nat Rev Microbiol, doi: 10.1038/nrmicro2839

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Authors and Affiliations

  1. Julius-von-Sachs-Institut für Biowissenschaften, Universität Würzburg, Julius-von-Sachs-Platz 3, D-97082, Würzburg, Germany

    Kristina Bayer & Ute Hentschel

Authors
  1. Kristina Bayer
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  2. Ute Hentschel
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Corresponding author

Correspondence to Kristina Bayer.

Additional information

Kristina Bayer 1997–2004 Biologiestudium an der Universität Würzburg. Dort 2008 Promotion am Zentrum für Infektionsforschung. Seit 2008 wissenschaftliche Assistentin am Julius-von-Sachs-Institut für Biowissenschaften, Universität Würzburg.

Ute Hentschel Humeida 1986–1988 Biologiestudium, Universität Hannover; 1988–1994 Studium der Meeresbiologie und Promotion, Scripps Institution of Oceanography, La Jolla, CA, USA. 1995–1997 Postdoc, University of California, Santa Barbara, USA. 1998–2003 Arbeitsgruppenleiterin, Institut für Molekulare Infektionsbiologie, Universität Würzburg. 2004 Habilitation und Nachwuchsgruppenleiterin, Zentrum für Infektionsforschung, Universität Würzburg. Seit 2008 Professorin für chemische Ökologie am Julius-von-Sachs-Institut für Biowissenschaften, Universität Würzburg.

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Bayer, K., Hentschel, U. Einzelzell-Genomik: das Bakterium als Individuum. Biospektrum 18, 596–598 (2012). https://doi.org/10.1007/s12268-012-0234-4

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  • DOI: https://doi.org/10.1007/s12268-012-0234-4

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