Analyse eines unkonventionellen Vitamin-Transporters
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Förster resonance energy transfer (FRET) identifies the co-localization of fluorescent macromolecules on a nanometer scale. FRET between nonidentical and identical fluorophores is called HeteroFRET or HomoFRET, respectively. We applied FRET techniques to analyze the oligomeric state of the core component (BioY) of a bacterial biotin transporter. BioY was fused to fluorescent proteins suitable for Hetero- and HomoFRET analyses. Spectrometric and imaging experiments with living cells strongly support a model with oligomers of BioY as the functional state of the core unit.
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- Kirsch F, Frielingsdorf S, Pohlmann A et al. (2012) Essential amino acid residues of BioY reveal that dimers are the functional S unit of the Rhodobacter capsulatus biotin transporter. J Bacteriol 194 (im Druck), doi: 10.1128/JB.00683-12Google Scholar