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BIOspektrum

, Volume 18, Issue 4, pp 390–393 | Cite as

Funktionale Zelllinien durch neue Immortalisierungsprotokolle

Zellsysteme
Wissenschaft · Special: Zellbiologi

Abstract

Cell lines are immortal cultures of cells that are used in research as well as for production of pharmaceuticals. Currently available cell lines are derived from normal and tumorous tissue of humans and diverse animal species, however, with considerable loss of specific properties. The development of new immortalization protocols has led to a significant improvement of the properties of the resulting cell lines. These retain specific properties and activities that include functional grafting.

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Literatur

  1. [1]
    Bodnar AG, Ouellette M, Frolkis M et al. (1998) Extension of life-span by introduction of telomerase into normal human cells. Science 279:349–352PubMedCrossRefGoogle Scholar
  2. [2]
    Simonsen JL, Rosada C, Serakinci N et al. (2002) Telomerase expression extends the proliferative life-span and maintains the osteogenic potential of human bone marrow stromal cells. Nat Biotechnol 20:592–596PubMedCrossRefGoogle Scholar
  3. [3]
    Yang J, Nagavarapu U, Relloma K et al. (2001) Telomerized human microvasculature is functional in vivo. Nat Biotechnol 19:219–224PubMedCrossRefGoogle Scholar
  4. [4]
    May T, Butueva M, Bantner S et al. (2010) Synthetic gene regulation circuits for control of cell expansion. Tissue Eng Part A 16:441–452PubMedCrossRefGoogle Scholar
  5. [5]
    Noguchi H, Kobayashi N, Westerman KA et al. (2002) Controlled expansion of human endothelial cell populations by Cre-loxP-based reversible immortalization. Hum Gene Ther 13:321–334PubMedCrossRefGoogle Scholar
  6. [6]
    Rybkin II, Markham DW, Yan Z et al. (2003) Conditional expression of SV40 T-antigen in mouse cardiomyocytes facilitates an inducible switch from proliferation to differentiation. J Biol Chem 278:15927–15934PubMedCrossRefGoogle Scholar
  7. [7]
    Westerman KA, Leboulch P (1996) Reversible immortalization of mammalian cells mediated by retroviral transfer and site-specific recombination. Proc Natl Acad Sci USA 93:8971–8976PubMedCrossRefGoogle Scholar
  8. [8]
    Botezatu L, Sievers S, Gama-Norton L et al. (2011) Genetic aspects of cell line development from a synthetic biology perspective. Adv Biochem Eng Biotechnol 127:251–284Google Scholar
  9. [9]
    May T, Eccleston L, Herrmann S et al. (2008) Bimodal and hysteretic expression in mammalian cells from a synthetic gene circuit. PLoS ONE 3:e2372PubMedCrossRefGoogle Scholar
  10. [10]
    May T, Hauser H, Wirth D (2004) Transcriptional control of SV40 T-antigen expression allows a complete reversion of immortalization. Nucleic Acids Res 32:5529–5538PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  1. 1.Inscreenex GMBHBraunschweigGermany
  2. 2.Helmholtz-Zentrum für InfektionsforschungBraunschweigGermany
  3. 3.Helmholtz-Zentrum für Infektionsforschung — HZIAbt. Genregulation und DifferenzierungBraunschweigGermany

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