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Schnelle Trennung von synthetischen RNAi-Sequenzen

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  • Ionenpaar-Umkehrphasen-UPLC
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Zusammenfassung

Unter Verwendung eines quaternären UPLC-Chromatographiesystems wurde die effiziente Trennung eines heterogenen Gemischs aus einem synthetischen RNAi-Oligonukleotid und dessen unterbrochenen Sequenzen erzielt.

Abstract

An efficient separation of a heterogeneous mixture of synthetic interfering RNAi oligonucleotide and its failed sequences was achieved using a quaternary type UPLC chromatography system.

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Literatur

  1. Gilar M, Fountain KJ, Budman Y et al. (2003) Characterization of therapeutic oligonucleotides using liquid chromatography with on-line mass spectrometry detection. Oligonucleotides 13:229–243

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  2. UPLC Separation of Oligonucleotides: Method Development, Part 1. Waters Technische Mitteilung 2007:720002383EN

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Author information

Authors and Affiliations

  1. Waters Corporation, Milford, MA, USA

    Vera B. Ivleva, Martin Gilar & Tom Wheat

  2. Waters GMBH, Eschborn, Germany

    Alexander Muck

  3. Waters GmbH, Helfmann-Park 10, D-65760, Eschborn, Germany

    Alexander Muck

Authors
  1. Vera B. Ivleva
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  2. Martin Gilar
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  3. Alexander Muck
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  4. Tom Wheat
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Corresponding author

Correspondence to Alexander Muck.

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Ivleva, V.B., Gilar, M., Muck, A. et al. Schnelle Trennung von synthetischen RNAi-Sequenzen. Biospektrum 17, 66–67 (2011). https://doi.org/10.1007/s12268-011-0014-6

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  • DOI: https://doi.org/10.1007/s12268-011-0014-6

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