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Inhibition of MicroRNA-206 Ameliorates Ischemia–Reperfusion Arrhythmia in a Mouse Model by Targeting Connexin43

  • Yan Jin
  • Tianyi Zhou
  • Qiuting Feng
  • Jun Yang
  • Jianing Cao
  • Xin Xu
  • Chengjian YangEmail author
Original Article

Abstract

Reperfusion arrhythmias (RA) are an important cause of sudden cardiac death and is closely associated with gap junction protein in the heart, connexin 43 (Cx43). This study is aimed at elucidating the molecular association between microRNA-206 (miR-206) and Cx43 in ischemia-reperfusion arrhythmia using experimental animal model. Our results showed that miR-206 inhibitor alleviated ischemia-reperfusion–induced arrhythmias, indicated by the lower extent of changes in heart rate (HR), PR interval, rate pressure product (RPP), and mean arterial pressure (MAP). miR-206 inhibitor also downregulated the serum creatine kinase isoenzyme (CKMB) and cardiac troponin I (cTnI) levels in mice under myocardial ischemia-reperfusion (IR) process. The knockdown of Cx43 inversed the protective effects of miR-206 inhibitor on cardiac arrhythmias. These results supported that inhibition of miR-206 ameliorates ischemia-reperfusion arrhythmia by targeting Cx43, and this miR-206/Cx43 axis could serve as a potential target for the management of ischemic-perfusion arrhythmia.

Keywords

Cardiac arrhythmias Ischemia-reperfusion Gap junction miR-206 Connexin 43 

Notes

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

Research Involving Human Participants and/or Animals

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

Informed Consent

Not applicable.

Supplementary material

12265_2019_9940_MOESM1_ESM.docx (304 kb)
ESM 1 (DOCX 303 kb)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Yan Jin
    • 1
  • Tianyi Zhou
    • 1
  • Qiuting Feng
    • 1
  • Jun Yang
    • 1
  • Jianing Cao
    • 1
  • Xin Xu
    • 1
  • Chengjian Yang
    • 1
    Email author
  1. 1.Department of CardiologyThe Affiliated Wuxi No.2 People’s Hospital of Nanjing Medical UniversityWuxiChina

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