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MicroRNA-29b-3p Targets SPARC Gene to Protect Cardiocytes against Autophagy and Apoptosis in Hypoxic-Induced H9c2 Cells

  • Shu Zhou
  • Dazhou Lei
  • Faqin Bu
  • Hongqiang Han
  • Shucai Zhao
  • Yan Wang
Original Article
  • 52 Downloads

Abstract

MicroRNAs participate in the regulation of abnormal cardiomyocyte apoptosis and autophagy, which leads to heart failure (HF). Lower miR-29b-3p levels were found in HF patients in this study. However, the role of miR-29b-3p in the molecular pathogenesis of HF remains unclear. Hypoxia-stimulated H9c2 cells were used an in vitro model of HF. It was found that hypoxia stimulation decreased the miR-29b-3p expression and enhanced cell apoptosis and autophagy response in H9c2 cells. While the effects of hypoxia on cell apoptosis and autophagy were reversed by miR-29b-3p transfection, especially 100 nM. The secreted protein acidic and rich in cysteine (SPARC), predicted as a direct target of miR-29b-3p, aggravated the hypoxia-induced cells apoptosis, autophagy, and TGFβ1/Smad3 activation. While the changes were dramatically reversed by miR-29b-3p. Taken together, our data suggest that miR-29b-3p plays an important role in the progression of HF through targeting SPARC and regulating TGFβ1/Smad3 pathway.

Keywords

MicroRNA-29b-3p Heart failure Secreted protein acidic and rich in cysteine Apoptosis Autophagy 

Notes

Acknowledgements

The authors would like to thank the members of the Second Department of Cardiology, Xinxiang Central Hospital for participating and providing blood samples in this study.

Compliance with Ethical Standards

Conflict of Interest

The author declares that they have no conflicts of interest.

Human Subjects/Informed Consent Statement

The present study was approved by the Ethics Committee of Xinxiang Central Hospital, and written informed consent was obtained from the patients and healthy donors.

Animal Studies

No animal studies were carried out by the authors for this article.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Shu Zhou
    • 1
  • Dazhou Lei
    • 1
  • Faqin Bu
    • 1
  • Hongqiang Han
    • 1
  • Shucai Zhao
    • 1
  • Yan Wang
    • 1
  1. 1.Second Department of CardiologyXinxiang Central HospitalXinxiangChina

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