Serum Extracellular Vesicles Retard H9C2 Cell Senescence by Suppressing miR-34a Expression
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Extracellular vesicles (EVs) are small-sized membrane-surrounded structures released from cells into the blood, which play important roles in regulating various biological processes. However, the role of EVs in Doxorubicin (DOX)-induced cardiomyocytes senescence remains elusive. In this study, we found that human serum EVs inhibited DOX-induced senescence in H9C2 cells, which was abolished by miR-34a mimic. Our study also proved that miR-34a mediated DOX-induced H9C2 cell senescence by targeting phosphatase 1 nuclear targeting subunit (PNUTS). In addition to the downregulation of miR-34a, EVs could upregulate the expression of PNUTS. Moreover, the inhibitory effect of serum EVs on DOX-induced H9C2 cell senescence was also impeded by PNUTS siRNA. In conclusion, our study suggests that serum EVs retard H9C2 cell senescence through the miR-34a/PNUTS pathway, providing a potential therapy for cardiac aging.
KeywordsSerum extracellular vesicles Cell senescence miR-34a Phosphatase 1 nuclear targeting subunit
This work was supported by the grants from National Natural Science Foundation of China (81470515, 81670362, and 81600228) and Shanghai Medical Guide Project from Shanghai Science and Technology Committee (134119a3000).
Compliance with Ethical Standards
Conflict of Interest
The authors declare that they have no conflict of interest.
Research Involving Human Participants and/or Animals
All human investigations conformed to the principles outlined in the Declaration of Helsinki and were approved by the institutional review committees of Shanghai Tongji Hospital.
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