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miRNA Profiling of Exosomes from Spontaneous Hypertensive Rats Using Next-Generation Sequencing

  • Xiaoyan Liu
  • Wen Yuan
  • Lei Yang
  • Jing Li
  • Jun CaiEmail author
Article

Abstract

The role and miRNA expression profile of exosomes in hypertension remain largely unknown. Therefore, next-generation sequencing was used to define the miRNA expression profile of plasma exosomes in spontaneously hypertensive rats (SHRs), the most widely used animal model of human essential hypertension, and their controls, normotensive Wistar-Kyoto rats (WKYs). Results revealed that percentages of miRNA in the total small RNA isolated from SHRs and WKYs were not significantly different. Twenty-seven miRNAs were significantly differentially expressed (DE) between SHR and WKY exosomes, including 23 up-regulated and four down-regulated in SHR exosomes as compared to WKY exosomes. Gene Ontology analysis and Kyoto Encyclopedia of Genes and Genomes pathway analysis of top 10 DE miRNAs identified hypertension-specific target genes/signaling pathways. In conclusion, our findings indicated the selective packing of miRNA cargo into exosomes under hypertensive status, which could facilitate the development of potential targets for the diagnosis, prevention, and treatment of hypertension.

Keywords

Exosomes Hypertension miRNA 

Abbreviations

NGS

Next-generation sequencing

SHRs

Spontaneously hypertensive rats

WKYs

Wistar-Kyoto rats

GO

Gene Ontology

KEGG

Kyoto Encyclopedia of Genes and Genomes

EDTA

Ethylenediaminetetraacetic acid

DE

Differentially expressed

CTGs

Candidate target genes

SBP

Systolic blood pressure

DBP

Diastolic blood pressure

HR

Heart rate

Notes

Sources of Funding

This work was supported by National Natural Science Foundation of China (81470541, 81500038, 81630014), National Basic Research Program of China (973 Program, 2014CB542302), CAMS Innovation Fund for Medical Sciences(CIFMS, 2016-12M-1-006), Beijing Municipal Science and Technology Commission (Z151100002115050, Z151100004015176), Beijing Municipal Commission of Education (KZ201610025028), and Beijing Municipal Natural Science Foundation (7172078).

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

Human Subjects/Informed Consent Statement

No human studies were conducted.

Ethical Approval of Animal Studies

All institutional and national guidelines for the care and use of laboratory animals were followed and approved by the appropriate institutional committees.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Xiaoyan Liu
    • 1
    • 2
  • Wen Yuan
    • 1
  • Lei Yang
    • 1
  • Jing Li
    • 2
  • Jun Cai
    • 3
    Email author
  1. 1.Medical Research Center, Beijing Chaoyang HospitalCapital Medical UniversityBeijingChina
  2. 2.Heart Center & Beijing Key Laboratory of Hypertension Research, Beijing Chaoyang HospitalCapital Medical UniversityBeijingChina
  3. 3.Hypertension Center of Fuwai Hospital, State Key Laboratory of Cardiovascular Disease of China, National Center for Cardiovascular Diseases of ChinaChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingChina

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