Systemic Delivery of Nanoparticles Loaded with Pentagalloyl Glucose Protects Elastic Lamina and Prevents Abdominal Aortic Aneurysm in Rats
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Degeneration of elastin plays a vital role in the pathology and progression of abdominal aortic aneurysm (AAA). Our previous study showed that pentagalloyl glucose (PGG), a core derivative of tannic acid, hinders the development of AAAs in a clinically relevant animal model when applied locally. In this study, we tested whether targeted nanoparticles (NPs) can deliver PGG to the site of an aneurysm and prevent aneurysmal growth by protecting elastin. PGG-loaded albumin NPs with a surface-conjugated elastin-specific antibody were prepared. Aneurysms were induced by calcium chloride-mediated injury to the abdominal aorta in rats. NPs were injected into the tail vein after 10 days of CaCl2 injury. Rats were euthanized after 38 days. PGG delivery led to reduction in macrophage recruitment, matrix metalloproteinase (MMP) activity, elastin degradation, calcification, and development of aortic aneurysm. Such NP delivery offers the potential for the development of effective and safe therapies for AAA.
KeywordsElastin stabilization and regeneration Matrix metalloproteinase inhibition
Abdominal aortic aneurysm
Bovine serum albumin
Elastin antibody-conjugated blank NPs
Elastin antibody-conjugated and DIR dye-loaded NPs
Elastin antibody-conjugated and PGG-loaded NPs
Compliance with Ethical Standards
Conflict of Interest
Last author has received research grants from NIH and Hunter Endowment at Clemson University. Other authors have no conflict of interest.
The study is financially supported by NIH grant P20GM103444 and the Hunter Endowment at Clemson University to (NV).
Human and Animal Rights and Informed Consent
This article does not contain any studies with human participants performed by any of the authors. Clemson University Animal Research Committee approves all animal use protocols (AUP) for the experimental models. All animals receive humane care in compliance with NIH Public Law 99-158, November 20, 1985, “Animals in Research,” revised in 2015.
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