Standard Operating Procedures (SOPs) for Evaluating the Heart in Preclinical Studies of Duchenne Muscular Dystrophy

  • Dongsheng Duan
  • Jill A. Rafael-Fortney
  • Alison Blain
  • David A. Kass
  • Elizabeth M. McNally
  • Joseph M. Metzger
  • Christopher F. Spurney
  • Kathi Kinnett


A recent working group meeting focused on contemporary cardiac issues in Duchenne muscular dystrophy (DMD) was hosted by the National Heart, Lung, and Blood Institute in collaboration with the Parent Project Muscular Dystrophy. An outcome of this meeting was to provide freely available detailed protocols for preclinical animal studies. The goal of these protocols is to improve the quality and reproducibility of cardiac preclinical studies aimed at developing new therapeutics for the prevention and treatment of DMD cardiomyopathy.


Duchenne Cardiology Cardiomyopathy Standard operating procedures (SOPs) Preclinical 



The authors thank the National Heart, Lung, and Blood Institute and the Parent Project Muscular Dystrophy.

Compliance with Ethical Standards

Conflict of Interest

D.D. is a member of the scientific advisory board for Solid GT, a subsidiary of Solid Biosciences. The Duan lab received research support from Solid Biosciences in 2014 and 2015. The other authors report no conflicts.


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Dongsheng Duan
    • 1
    • 2
  • Jill A. Rafael-Fortney
    • 3
  • Alison Blain
    • 4
  • David A. Kass
    • 5
  • Elizabeth M. McNally
    • 6
  • Joseph M. Metzger
    • 7
  • Christopher F. Spurney
    • 8
  • Kathi Kinnett
    • 9
  1. 1.Department of Molecular Microbiology and Immunology, School of MedicineUniversity of MissouriColumbiaUSA
  2. 2.Department of Neurology, School of MedicineUniversity of MissouriColumbiaUSA
  3. 3.Departments of Physiology & Cell Biology and Molecular & Cellular Biochemistry, College of MedicineThe Ohio State UniversityColumbusUSA
  4. 4.Institute of Genetic MedicineNewcastle UniversityNewcastleUK
  5. 5.The Johns Hopkins University Medical InstitutionsBaltimoreUSA
  6. 6.Center for Genetic MedicineNorthwestern University Feinberg School of MedicineChicagoUSA
  7. 7.Department of Integrative Biology and PhysiologyUniversity of Minnesota Medical SchoolMinneapolisUSA
  8. 8.Division of Cardiology and Center for Genetic Medicine Research, Children’s National Heart InstituteChildren’s National Health SystemWashingtonUSA
  9. 9.Parent Project Muscular DystrophyMiddletownUSA

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