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N-acetylcysteine Plus Deferoxamine Improves Cardiac Function in Wistar Rats After Non-reperfused Acute Myocardial Infarction

  • Amanda Phaelante
  • Luís Eduardo Rohde
  • Amanda Lopes
  • Virgílio Olsen
  • Santiago Alonso Leitão Tobar
  • Carolina Cohen
  • Nidiane Martinelli
  • Andréia Biolo
  • Felipe Dal-Pizzol
  • Nadine Clausell
  • Michael AndradesEmail author
Article

Abstract

The antioxidant N-acetycysteine can turn into a prooxidant molecule in presence of iron ions. Thus, our goal was to test if the association of N-acetylcysteine (NAC) and an iron chelator (deferoxamine—DFX) in a rodent model of acute myocardial infarction (AMI) improves cardiac function. Male Wistar rats were subjected to a SHAM surgery or AMI. The animals were randomized: vehicle, NAC (25 mg/kg for 28 days), DFX (40 mg/kg for 7 days), or NAC plus DFX (NAC plus DFX, respectively). Animals were killed 28 days after the AMI. Animals treated with NAC/DFX showed an increase in left ventricular ejection fraction at 28 days when compared with vehicle group (45.2 ± 10.9 % vs. 34.7 ± 8.7 %, p = 0.03). Antioxidant effect of NAC/DFX treatment decreased 4-hydroxynonenal when compared to AMI group (p = 0.06). In conclusion, we showed beneficial effect of NAC/DFX association in improving left ventricle function in an experimental AMI.

Keywords

N-acetylcysteine Deferoxamine Heart failure Myocardial infarction Oxidative stress 

Abbreviations

AMI

Acute myocardial infarction

DFX

Deferoxamine

EF

Ejection fraction

FS

Fractional shortening

HNE

Hydroxynonenal

LV

Left ventricular

LVDD

Left ventricular diastolic diameter

LVSD

Left ventricular systolic diameter

NAC

N-acetylcysteine

PE

Perimeter of the endocardium

ROS

Reactive oxygen species

Notes

Funding

This work was supported in part by grants from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Secretaria de Ciência e Tecnologia da Fundação de Amparo a Pesquisa do Estado do Rio Grande do Sul (FAPERGS # 11/1970-3), and Fundo de Incentivo à Pesquisa e Eventos do Hospital de Clínicas de Porto Alegre (FIPE-HCPA).

Animal Study

No human studies were carried out by the authors for this article.

All procedures followed during this investigation are in accordance with the Guide for the Care and Use of Laboratory Animals published by the US National Institutes of Health (NIH Publication No. 85-23, revised 1996), and the experimental protocol was approved by the Ethics Committee of the Hospital de Clinicas de Porto Alegre (CEUA) by the number 110202.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Amanda Phaelante
    • 1
    • 2
  • Luís Eduardo Rohde
    • 1
    • 2
  • Amanda Lopes
    • 1
  • Virgílio Olsen
    • 1
  • Santiago Alonso Leitão Tobar
    • 1
  • Carolina Cohen
    • 1
  • Nidiane Martinelli
    • 1
  • Andréia Biolo
    • 1
    • 2
  • Felipe Dal-Pizzol
    • 3
  • Nadine Clausell
    • 1
    • 2
  • Michael Andrades
    • 1
    • 2
    Email author
  1. 1.Cardiovascular Research Laboratory, Experimental Research CenterHospital de Clínicas de Porto AlegrePorto AlegreBrazil
  2. 2.Post-Graduate Program in Cardiology and Cardiovascular ScienceUniversidade Federal do Rio Grande do SulPorto AlegreBrazil
  3. 3.Departamento de MedicinaUniversidade do Extremo Sul CatarinenseCriciúmaBrazil

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