Association of Genetic Variation with Gene Expression and Protein Abundance within the Natriuretic Peptide Pathway

  • David E. LanfearEmail author
  • Bipin Sunkara
  • Jia Li
  • Sharad Rastogi
  • Ramesh C. Gupta
  • Badri Padhukasahasram
  • L. Keoki Williams
  • Hani N. Sabbah


The natriuretic peptide (NP) system is a critical physiologic pathway in heart failure with wide individual variability in functioning. We investigated the genetic component by testing the association of single nucleotide polymorphisms (SNP) with RNA and protein expression. Samples of DNA, RNA, and tissue from human kidney (n = 103) underwent genotyping, RT-PCR, and protein quantitation (in lysates), for four candidate genes [NP receptor 1 (NPR1), NPR2, and NPR3 and membrane metalloendopeptidase]. The association of genetic variation with expression was tested using linear regression for individual SNPs, and a principal components (PC) method for overall gene variation. Eleven SNPs in NPR2 were significantly associated with protein expression (false discovery rate ≤0.05), but not RNA quantity. RNA and protein quantity correlated poorly with each other. The PC analysis showed only NPR2 as significant. Assessment of the clinical impact of NPR2 genetic variation is needed.


Natriuretic peptide Heart failure Gene expression Pharmacogenomics Nesiritide Genetic polymorphisms 



We thank the Alvin J. Siteman Cancer Center at Washington University School of Medicine and Barnes-Jewish Hospital in St. Louis, MO, USA, for the use of the Tissue Procurement Core, which provided tissue, DNA, and RNA isolation services. The Siteman Cancer Center is supported in part by a NCI Cancer Center Support Grant #P30 CA91842. This work was supported by grants from the National Institutes of Health (Lanfear, HL085124 and HL103871).

Conflicts of interest

The authors declare that they have no competing interests.

Supplementary material

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ESM 1 (DOCX 24 kb)


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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • David E. Lanfear
    • 1
    • 3
    Email author
  • Bipin Sunkara
    • 1
  • Jia Li
    • 2
  • Sharad Rastogi
    • 1
  • Ramesh C. Gupta
    • 1
  • Badri Padhukasahasram
    • 3
  • L. Keoki Williams
    • 3
  • Hani N. Sabbah
    • 1
  1. 1.Department of Internal Medicine, Division of Cardiology, Henry Ford Heart and Vascular InstituteHenry Ford HospitalDetroitUSA
  2. 2.Public Health SciencesHenry Ford HospitalDetroitUSA
  3. 3.Center for Health Services and Policy ResearchHenry Ford HospitalDetroitUSA

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