Tissue-Engineered Heart Valves: Intra-operative Protocol
- 298 Downloads
Tissue engineering of heart valves investigates the possibility to create a fully compatible and biomimetic graft able to provide host cell repopulation like the native living valve. Decellularized aortic and pulmonary valves and synthetic polymers have been used to promote the creation of a native-like scaffold suitable to be colonized by cells either in vitro, in dynamic bioreactors or in vivo using different animal models. The herein presented research provides the intra-operative protocol and details of surgical technique. Porcine aortic valve conduits were decellularized and implanted in the right ventricular outflow tract of Vietnamese pigs.
KeywordsTissue engineering Surgical technique Heart valves Decellularization Animal model
(MPG 26004 kb)
- 1.Spina, M., Ortolani, F., El Messlemani, A., Gandaglia, A., Bujan, J., Garcia-Honduvilla, N., et al. (2003). Isolation of intact aortic valve scaffolds for heart-valve bioprostheses: extracellular matrix structure, prevention from calcification, and cell repopulation features. Journal of Biomedical Materials Research. Part A, 67, 1338–1350.PubMedGoogle Scholar
- 4.Gallo, M., Naso, F., Poser, H., Bianco, R., Franci, P., Micciolo, M., et al. (2012 Jun). In vivo preliminary findings of a tissue engineered heart valve implanted for 15 months in a Vietnamese pig. Artificial Organs, 36(6), E138–50.Google Scholar