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Tissue-Engineered Heart Valves: Intra-operative Protocol

  • Michele GalloEmail author
  • Roberto Bianco
  • Tomaso Bottio
  • Filippo Naso
  • Paolo Franci
  • Fabio Zanella
  • Giovanni Perona
  • Roberto Busetto
  • Michele Spina
  • Alessandro Gandaglia
  • Gino Gerosa
Article

Abstract

Tissue engineering of heart valves investigates the possibility to create a fully compatible and biomimetic graft able to provide host cell repopulation like the native living valve. Decellularized aortic and pulmonary valves and synthetic polymers have been used to promote the creation of a native-like scaffold suitable to be colonized by cells either in vitro, in dynamic bioreactors or in vivo using different animal models. The herein presented research provides the intra-operative protocol and details of surgical technique. Porcine aortic valve conduits were decellularized and implanted in the right ventricular outflow tract of Vietnamese pigs.

Keywords

Tissue engineering Surgical technique Heart valves Decellularization Animal model 

Supplementary material

ESM 1

(MPG 26004 kb)

References

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Michele Gallo
    • 1
    Email author
  • Roberto Bianco
    • 1
  • Tomaso Bottio
    • 1
  • Filippo Naso
    • 1
  • Paolo Franci
    • 2
  • Fabio Zanella
    • 1
  • Giovanni Perona
    • 3
  • Roberto Busetto
    • 2
  • Michele Spina
    • 4
  • Alessandro Gandaglia
    • 1
  • Gino Gerosa
    • 1
  1. 1.Division of Cardiac Surgery, Department of Cardiac, Thoracic and Vascular ScienceUniversity of PaduaPaduaItaly
  2. 2.Clinical Veterinary ScienceUniversity of PaduaPaduaItaly
  3. 3.Department of Veterinary ScienceUniversity of TurinTurinItaly
  4. 4.Experimental Biomedical SciencesUniversity of PaduaPaduaItaly

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