Association of Biomarkers of Lipid Modification with Functional and Morphological Indices of Coronary Stenosis Severity in Stable Coronary Artery Disease

  • Olivier Muller
  • Argyrios Ntalianis
  • William Wijns
  • Leen Delrue
  • Karen Dierickx
  • Reto Auer
  • Nicolas Rodondi
  • Fabio Mangiacapra
  • Catalina Trana
  • Michalis Hamilos
  • Emmanuel Valentin
  • Bernard De Bruyne
  • Emanuele Barbato
  • Jozef Bartunek


Biomarkers of blood lipid modification and oxidative stress have been associated with increased cardiovascular morbidity. We sought to determine whether these biomarkers were related to functional indices of stenosis severity among patients with stable coronary artery disease. We studied 197 consecutive patients with stable coronary artery disease due to single vessel disease. Fractional flow reserve (FFR) ≤ 0.80 was assessed as index of a functionally significant lesion. Serum levels of secretory phospholipase A2 (sPLA2) activity, secretory phospholipase A2 type IIA (sPLA2-IIA), myeloperoxydase (MPO), lipoprotein-associated phospholipase A2 (Lp-PLA2), and oxidized low-density lipoprotein (OxLDL) were assessed using commercially available assays. Patients with FFR > 0.8 had higher sPLA2 activity, sPLA2 IIA, and OxLDL levels than patients with FFR ≤ 0.8 (21.25 [16.03–27.28] vs 25.85 [20.58–34.63] U/mL, p < 0.001, 2.0 [1.5–3.4] vs 2.6 [2.0–3.4] ng/mL, p < 0.01; and 53.0 [36.0–71.0] vs 64.5 [50–89.25], p < 0.001 respectively). Patients with FFR > 0.80 had similar Lp-PLA2 and MPO levels versus those with FFR ≤ 0.8. sPLA2 activity, sPLA2 IIA significantly increased area under the curve over baseline characteristics to predict FFR ≤ 0.8 (0.67 to 0.77 (95 % confidence interval [CI]: 0.69–0.85) p < 0.01 and 0.67 to 0.77 (95 % CI: 0.69–0.84) p < 0.01, respectively). Serum sPLA2 activity as well as sPLA2-IIA level is related to functional characteristics of coronary stenoses in patients with stable coronary artery disease.


Biomarkers of lipid modifications Fractional flow reserve Phospolipase 



Secretory phospholipase A2 activity


Secretory phospholipase A2 activity type IIA




Lipoprotein-associated phospholipase A2


Oxidized low-density lipoprotein


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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Olivier Muller
    • 1
    • 2
  • Argyrios Ntalianis
    • 1
  • William Wijns
    • 1
  • Leen Delrue
    • 1
  • Karen Dierickx
    • 1
  • Reto Auer
    • 3
  • Nicolas Rodondi
    • 4
  • Fabio Mangiacapra
    • 1
  • Catalina Trana
    • 1
  • Michalis Hamilos
    • 1
  • Emmanuel Valentin
    • 5
  • Bernard De Bruyne
    • 1
  • Emanuele Barbato
    • 1
  • Jozef Bartunek
    • 1
    • 6
  1. 1.Cardiovascular Center and Translational Cardiology UnitAalstBelgium
  2. 2.Interventional Cardiology UnitDepartment of Cardiology, Centre Hospitalier Universitaire VaudoisLausanneSwitzerland
  3. 3.Department of Epidemiology and BiostatisticsUniversity of CaliforniaSan FranciscoUSA
  4. 4.Department of General Internal MedicineUniversity of BernBernSwitzerland
  5. 5.AterovaxParisFrance
  6. 6.Cardiovascular Center and Cardiovascular Research CenterOLV HospitalAalstBelgium

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