Attenuation of Monocyte Chemotaxis—A Novel Anti-inflammatory Mechanism of Action for the Cardio-protective Hormone B-Type Natriuretic Peptide

  • Nadezhda Glezeva
  • Patrick Collier
  • Victor Voon
  • Mark Ledwidge
  • Kenneth McDonald
  • Chris Watson
  • John Baugh


B-type natriuretic peptide (BNP) is a prognostic and diagnostic marker for heart failure (HF). An anti-inflammatory, cardio-protective role for BNP was proposed. In cardiovascular diseases including pressure overload-induced HF, perivascular inflammation and cardiac fibrosis are, in part, mediated by monocyte chemoattractant protein (MCP)1-driven monocyte migration. We aimed to determine the role of BNP in monocyte motility to MCP1. A functional BNP receptor, natriuretic peptide receptor-A (NPRA) was identified in human monocytes. BNP treatment inhibited MCP1-induced THP1 (monocytic leukemia cells) and primary monocyte chemotaxis (70 and 50 %, respectively). BNP did not interfere with MCP1 receptor expression or with calcium. BNP inhibited activation of the cytoskeletal protein RhoA in MCP1-stimulated THP1 (70 %). Finally, BNP failed to inhibit MCP1-directed motility of monocytes from patients with hypertension (n = 10) and HF (n = 6) suggesting attenuation of this anti-inflammatory mechanism in chronic heart disease. We provide novel evidence for a direct role of BNP/NPRA in opposing human monocyte migration and support a role for BNP as a cardio-protective hormone up-regulated as part of an adaptive compensatory response to combat excess inflammation.


BNP NPRA RhoA Monocyte chemotaxis Calcium Hypertension Heart failure 



We are greatly thankful to Dr. Alfonso Blanco for his instruction and assistance with the calcium measurements. This work was funded by the Irish Research Council for Science, Engineering and Technology and the Health Research Board of Ireland (Dublin, Ireland).

Conflicts of Interest

The authors confirm that there are no conflicts of interest.

Supplementary material

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ESM 1 (DOCX 252 kb)


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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Nadezhda Glezeva
    • 1
  • Patrick Collier
    • 1
    • 2
  • Victor Voon
    • 2
  • Mark Ledwidge
    • 2
  • Kenneth McDonald
    • 1
    • 2
  • Chris Watson
    • 1
    • 2
  • John Baugh
    • 1
  1. 1.School of Medicine and Medical Science, UCD Conway Institute for Biomolecular and Biomedical ResearchUniversity College DublinDublin 4Ireland
  2. 2.Heart Failure UnitSt. Vincent’s University Hospital Healthcare GroupDublinIreland

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