Transcriptome Analysis in Patients with Progressive Coronary Artery Disease: Identification of Differential Gene Expression in Peripheral Blood

  • Thomas G. Nührenberg
  • Nicole Langwieser
  • Harald Binder
  • Thorsten Kurz
  • Christian Stratz
  • Rolf-Peter Kienzle
  • Dietmar Trenk
  • Dietlind Zohlnhöfer-Momm
  • Franz-Josef Neumann
Article

Abstract

Inflammation as a systemic process plays a central role in atherosclerotic plaque progression (PP). Here we investigated other systemic correlates of PP by global gene expression profiling (GEP) in peripheral blood. From a database of 45,727 coronary angiograms, we identified two patient groups with good risk factor control, but different clinical evolution: First, 16 patients had significant PP leading to repeated coronary interventions, and second, 16 patients had angiographically documented stable courses. GEP revealed 93 differentially expressed genes, of which 23 have unknown function. Among the remaining 70 genes, 10 were associated with progenitor and pluripotent cells, but only three genes with atherosclerosis. We developed a risk prediction gene signature by a multivariable statistical model integrating comprehensive laboratory and clinical patient data. This signature identified PP with high sensitivity and specificity for new patients, as estimated by resampling techniques. GEP results were validated by qPCR for ANK2 and GSTT1.

Keywords

Coronary artery disease Atherosclerosis Plaque progression Gene expression 

Supplementary material

12265_2012_9420_MOESM1_ESM.ppt (120 kb)
ESM 1(PPT 119 kb)

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Copyright information

© Springer Science+Business Media New York 2012

Authors and Affiliations

  • Thomas G. Nührenberg
    • 1
  • Nicole Langwieser
    • 2
    • 3
  • Harald Binder
    • 4
    • 5
  • Thorsten Kurz
    • 6
  • Christian Stratz
    • 1
  • Rolf-Peter Kienzle
    • 1
  • Dietmar Trenk
    • 1
  • Dietlind Zohlnhöfer-Momm
    • 2
    • 3
  • Franz-Josef Neumann
    • 1
  1. 1.Klinik für Kardiologie und Angiologie IIUniversitäts-Herzzentrum Freiburg • Bad KrozingenBad KrozingenGermany
  2. 2.Medizinische Klinik mit Schwerpunkt Kardiologie, Campus Virchow KlinikumCharitéBerlinGermany
  3. 3.Berlin-Brandenburg Center for Regenerative TherapiesBerlinGermany
  4. 4.Institut für Medizinische Biometrie, Epidemiologie und InformatikUniversitätsmedizin der Johannes-Gutenberg-Universität MainzMainzGermany
  5. 5.Institut für Medizinische Biometrie und Medizinische InformatikUniversitätsklinikum FreiburgFreiburgGermany
  6. 6.Zentrum für Biosystemanalyse der Universität FreiburgFreiburgGermany

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