Enhanced Endothelialization of a New Stent Polymer Through Surface Enhancement and Incorporation of Growth Factor-Delivering Microparticles

  • Hao Xu
  • Kytai T. Nguyen
  • Emmanouil S. Brilakis
  • Jian Yang
  • Eric Fuh
  • Subhash Banerjee
Article
First Online:
Received:
Accepted:

Abstract

In this study, we sought to develop strategies for improved endothelialization of a new polymer coating for vascular stents. Surface enhancement of the new poly-1,8-octanediol-co-citric acid (POC) polymer was achieved through conjugation of anti-CD34 antibody and incorporation of vascular endothelial growth factor and basic fibroblast growth factor-containing poly-lactic-co-glycolic acid microparticles to improve capture and proliferation of endothelial progenitor cells (EPC) and compared to untreated POC and poly-L-lactic acid (PLLA) polymer. Our results indicate that compared to PLLA, POC coating was more hemocompatible, with less platelet activation (p = 0.01), thrombogenicity (p < 0.05 for 20 and 30 min clot formation), and inflammatory response (IL-1β release, p = 0.0009; TNF-α release, p = 0.004). EPC adhesion and proliferation on POC were significantly improved with surface enhancement and microparticle incorporation compared to untreated POC (p = 0.006) and PLLA (p = 0.003). These results suggest a new strategy for enhancing endothelialization of polymeric coatings of vascular prostheses.

Keywords

Endothelialization Endothelial progenitor cells Poly-1,8-octanediol-co-citric acid polymer Microparticle 
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Notes

Acknowledgments

We acknowledge the support from the NIH (HL082644: K.N.) and UTA Research Area Enhancement grant.

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Copyright information

© Springer Science+Business Media, LLC (outside the USA) 2012

Authors and Affiliations

  • Hao Xu
    • 1
  • Kytai T. Nguyen
    • 2
  • Emmanouil S. Brilakis
    • 1
    • 3
  • Jian Yang
    • 2
  • Eric Fuh
    • 1
  • Subhash Banerjee
    • 1
    • 3
  1. 1.University of Texas Southwestern Medical CenterDallasUSA
  2. 2.University of Texas at ArlingtonArlingtonUSA
  3. 3.North Texas Veterans Affairs Medical CenterDallasUSA

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