From Molecules to Myofibers: Multiscale Imaging of the Myocardium
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Pathology in the heart can be examined at several scales, ranging from the molecular to the macroscopic. Traditionally, fluorescence-based techniques such as flow cytometry have been used to study the myocardium at the molecular, cellular, and microscopic levels. Recent advances in magnetic resonance imaging (MRI), however, have made it possible to image certain cellular and molecular events in the myocardium noninvasively in vivo. In addition, diffusion MRI has been used to image myocardial fiber architecture and microstructure in the intact heart. Diffusion MRI tractography, in particular, is providing novel insights into myocardial microsctructure in both health and disease. Recent developments have also been made in fluorescence imaging, making it possible to image fluorescent probes in the heart of small animals noninvasively in vivo. Moreover, techniques have been developed to perform in vivo fluorescence tomography of the mouse heart. These advances in MRI and fluorescence imaging allow events in the myocardium to be imaged at several scales linking molecular changes to alterations in microstructure and microstructural changes to gross function. A complete and integrated picture of pathophysiology in the myocardium is thus obtained. This multiscale approach has the potential to be of significant value not only in preclinical research but, ultimately, in the clinical arena as well.
KeywordsMRI Myocardium Molecular imaging Microstructure Fluorescence Diffusion MRI
Dr. Sosnovik has been funded in part by the following National Institutes of Health grant: R01 HL093038.
- 7.Sosnovik, D. E., Garanger, E., Aikawa, E., Nahrendorf, M., Figuiredo, J. L., Dai, G., et al. (2009). Molecular MRI of cardiomyocyte apoptosis with simultaneous delayed-enhancement MRI distinguishes apoptotic and necrotic myocytes in vivo: Potential for midmyocardial salvage in acute ischemia. Circulation Cardiovascular Imaging, 2(6), 460–467.PubMedCrossRefGoogle Scholar
- 23.Nahrendorf, M., Swirski, F. K., Aikawa, E., Stangenberg, L., Wurdinger, T., Figueiredo, J. L., et al. (2007). The healing myocardium sequentially mobilizes two monocyte subsets with divergent and complementary functions. The Journal of Experimental Medicine, 204(12), 3037–3047.PubMedCrossRefGoogle Scholar
- 26.Nahrendorf, M., Sosnovik, D., Chen, J. W., Panizzi, P., Figueiredo, J. L., Aikawa, E., et al. (2008). Activatable magnetic resonance imaging agent reports myeloperoxidase activity in healing infarcts and noninvasively detects the antiinflammatory effects of atorvastatin on ischemia–reperfusion injury. Circulation, 117(9), 1153–1160.PubMedCrossRefGoogle Scholar
- 31.Waters, E. A., Chen, J., Allen, J. S., Zhang, H., Lanza, G. M., & Wickline, S. A. (2008). Detection and quantification of angiogenesis in experimental valve disease with integrin-targeted nanoparticles and 19-fluorine MRI/MRS. Journal of Cardiovascular Magnetic Resonance, 10, 43.PubMedCrossRefGoogle Scholar
- 45.Tseng, W. Y., Reese, T. G., Weisskoff, R. M., & Wedeen, V. J. (1999). Cardiac diffusion tensor MRI in vivo without strain correction. Magnetic resonance in medicine: official journal of the Society of Magnetic Resonance in Medicine/Society of Magnetic Resonance in Medicine, 42(2), 393–403.Google Scholar
- 46.Gamper, U., Boesiger, P., & Kozerke, S. (2007). Diffusion imaging of the in vivo heart using spin echoes—considerations on bulk motion sensitivity. Magnetic resonance in medicine: official journal of the Society of Magnetic Resonance in Medicine/Society of Magnetic Resonance in Medicine, 57(2), 331–337.Google Scholar
- 47.Wu, M. T., Tseng, W. Y., Su, M. Y., Liu, C. P., Chiou, K. R., Wedeen, V. J., et al. (2006). Diffusion tensor magnetic resonance imaging mapping the fiber architecture remodeling in human myocardium after infarction: Correlation with viability and wall motion. Circulation, 114(10), 1036–1045.PubMedCrossRefGoogle Scholar
- 48.Wu, M. T., Su, M. Y., Huang, Y. L., Chiou, K. R., Yang, P., Pan, H. B., et al. (2009). Sequential changes of myocardial microstructure in patients postmyocardial infarction by diffusion-tensor cardiac MR: correlation with left ventricular structure and function. Circulation Cardiovascular imaging, 2(1), 32–40. 36 p following 40.PubMedCrossRefGoogle Scholar