MicroRNA-132 in the Adult Dentate Gyrus is Involved in Opioid Addiction Via Modifying the Differentiation of Neural Stem Cells
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MicroRNA-132 (miR-132), a small RNA that regulates gene expression, is known to promote neurogenesis in the embryonic nervous system and adult brain. Although exposure to psychoactive substances can increase miR-132 expression in cultured neural stem cells (NSCs) and the adult brain of rodents, little is known about its role in opioid addiction. So, we set out to determine the effect of miR-132 on differentiation of the NSCs and whether this effect is involved in opioid addiction using the rat morphine self-administration (MSA) model. We found that miR-132 overexpression enhanced the differentiation of NSCs in vivo and in vitro. Similarly, specific overexpression of miR-132 in NSCs of the adult hippocampal dentate gyrus (DG) during the acquisition stage of MSA potentiated morphine-seeking behavior. These findings indicate that miR-132 is involved in opioid addiction, probably by promoting the differentiation of NSCs in the adult DG.
KeywordsmiR-132 Opioid addiction Neural stem cell Dentate gyrus
This work was supported by grants from the National Natural Science Foundation (81471353 and 81771433), the National Basic Research Development Program of China (2015CB553500), and the Science Fund for Creative Research Groups from the National Natural Science Foundation of China (81521063).
Conflict of interest
The authors declare no competing interests.
- 7.Mathew RS, Tatarakis A, Rudenko A, Johnsonvenkatesh EM, Yang YJ, Murphy EA, et al. A microRNA negative feedback loop downregulates vesicle transport and inhibits fear memory. eLife 2016, 5. pii: e22467.Google Scholar
- 9.Aten S, Hansen KF, Hoyt KR, Obrietan K. The miR-132/212 locus: a complex regulator of neuronal plasticity, gene expression and cognition. RNA Dis 2016, 3. pii: e1375.Google Scholar
- 24.Walker TL, Kempermann G. One mouse, two cultures: isolation and culture of adult neural stem cells from the two neurogenic zones of individual mice. J Vis Exp 2014, 84: e51225.Google Scholar
- 33.Yue Z, Loh HH, Ping-Yee L. Effect of opioid on adult hippocampal neurogenesis. ScientificWorldJournal 2016, 2016: 2601264.Google Scholar
- 41.Bossert JM, Gray SM, Lu L, Shaham Y. Activation of group II metabotropic glutamate receptors in the nucleus accumbens shell attenuates context-induced relapse to heroin seeking. Neuropsychopharmacology 2006, 31: 2197–2209.Google Scholar
- 42.Yokel RA. Intravenous self-administration: response rates, the effects of pharmacological challenges, and drug preference. In: Bozarth MA (Ed.). Methods of Assessing the Reinforcing Properties of Abused Drugs. New York: Springer, 1987.Google Scholar
- 45.Ge F, Wang N, Cui C, Li Y, Liu Y, Ma Y, et al. Glutamatergic projections from the entorhinal cortex to dorsal dentate gyrus mediate context-induced reinstatement of heroin seeking. Neuropsychopharmacology 2017, 42: 1860.Google Scholar