Neuroscience Bulletin

, Volume 34, Issue 5, pp 849–853 | Cite as

Acute Restraint Stress Augments 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine Neurotoxicity via Increased Toxin Uptake into the Brain in C57BL/6 Mice

  • Yasuhide Mitsumoto
  • Atsushi Mori


As an environmental risk factor, psychological stress may trigger the onset or accelerate the progression of Parkinson’s disease (PD). Here, we evaluated the effects of acute restraint stress on striatal dopaminergic terminals and the brain metabolism of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), which has been widely used for creating a mouse model of PD. Exposure to 2 h of restraint stress immediately after injection of a low dose of MPTP caused a severe loss of striatal dopaminergic terminals as indicated by decreases in the dopamine transporter protein and dopamine levels compared with MPTP administration alone. Both striatal 1-methyl-4-phenylpyridinium ion (MPP+) and MPTP concentrations were significantly increased by the application of restraint stress. Striatal monoamine oxidase-B, which catalyzes the oxidation of MPTP to MPP+, was not changed by the restraint stress. Our results indicate that the enhanced striatal dopaminergic terminal loss in the stressed mice is associated with an increase in the transport of neurotoxin into the brain.


MPTP MPP+ Mouse model Restraint stress Dopaminergic neuron Degeneration Dopamine transporter Dopamine 



This work was supported by the Specific Research Foundation of Hokuriku University (250100).


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Copyright information

© Shanghai Institutes for Biological Sciences, CAS and Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  1. 1.Laboratory of Alternative Medicine and Experimental Therapeutics, Department of Clinical Pharmacy, Faculty of Pharmaceutical SciencesHokuriku UniversityKanazawaJapan
  2. 2.Department of Perioperative Management SystemNagoya University Graduate School of MedicineNagoyaJapan

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