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Neuroscience Bulletin

, Volume 28, Issue 5, pp 606–610 | Cite as

Curcumin protects against staurosporine toxicity in rat neurons

  • Xiao-Yan Qin
  • Ji-Hui Lv
  • Jia Cui
  • Xue Fang
  • Yan ZhangEmail author
Original Article

Abstract

Objective

Curcumin is extracted from the turmeric plant (Curcuma longa Linn.) and is widely used as a food additive and traditional medicine. The present study investigated the activity of curcumin against staurosporine (STS) toxicity in cell culture.

Methods

Rat hippocampal neurons in primary culture were exposed to STS (20 μmol/L) and treated with curcumin (20 μmol/L). Cell viability was tested by MTT assay and reactive oxygen species (ROS) were measured using the MitoSOX™ red mitochondrial superoxide indicator. Western blot was used to assess changes in the levels of caspase-3 (Csp3), heat shock protein 70 (Hsp70) and Akt.

Results

The results showed that curcumin protects against STS-induced cytotoxicity in rat hippocampal neurons. Csp3, Hsp70, Akt and ROS activation may be involved in this protection.

Conclusion

Curcumin could be a potential drug for combination with STS in cancer treatment to reduce the unwanted cytotoxicity of STS.

Keywords

curcumin staurosporine-induced cytotoxicity hippocampal neurons caspase-3 Akt Hsp70 reactive oxygen species cancer treatment 

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Copyright information

© Shanghai Institutes for Biological Sciences, CAS and Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Xiao-Yan Qin
    • 1
    • 2
  • Ji-Hui Lv
    • 3
  • Jia Cui
    • 1
    • 2
  • Xue Fang
    • 1
    • 2
  • Yan Zhang
    • 1
    • 2
    Email author
  1. 1.State Key Laboratory of Biomembrane and Membrane Biotechnolog, College of Life SciencesPeking UniversityBeijingChina
  2. 2.Laboratory of Biotechnology and State Key Laboratory of Chinese Ethnic Minority Traditional Medicine, College of Life & Environmental ScienceMinzu University of ChinaBeijingChina
  3. 3.Beijing Geriatric HospitalBeijingChina

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