Neuroscience Bulletin

, Volume 28, Issue 5, pp 606–610 | Cite as

Curcumin protects against staurosporine toxicity in rat neurons

  • Xiao-Yan Qin
  • Ji-Hui Lv
  • Jia Cui
  • Xue Fang
  • Yan ZhangEmail author
Original Article



Curcumin is extracted from the turmeric plant (Curcuma longa Linn.) and is widely used as a food additive and traditional medicine. The present study investigated the activity of curcumin against staurosporine (STS) toxicity in cell culture.


Rat hippocampal neurons in primary culture were exposed to STS (20 μmol/L) and treated with curcumin (20 μmol/L). Cell viability was tested by MTT assay and reactive oxygen species (ROS) were measured using the MitoSOX™ red mitochondrial superoxide indicator. Western blot was used to assess changes in the levels of caspase-3 (Csp3), heat shock protein 70 (Hsp70) and Akt.


The results showed that curcumin protects against STS-induced cytotoxicity in rat hippocampal neurons. Csp3, Hsp70, Akt and ROS activation may be involved in this protection.


Curcumin could be a potential drug for combination with STS in cancer treatment to reduce the unwanted cytotoxicity of STS.


curcumin staurosporine-induced cytotoxicity hippocampal neurons caspase-3 Akt Hsp70 reactive oxygen species cancer treatment 


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  1. [1]
    Omura S, Iwai Y, Hirano A, Nakagawa A, Awaya J, Tsuchya H, et al. A new alkaloid AM-2282 OF Streptomyces origin. Taxonomy, fermentation, isolation and preliminary characterization. J Antibiot (Tokyo) 1977, 30: 275–282.CrossRefGoogle Scholar
  2. [2]
    Takahashi I, Asano K, Kawamoto I, Tamaoki T, Nakano H. UCN-01 and UCN-02, new selective inhibitors of protein kinase C. I. Screening, producing organism and fermentation. J Antibiot (Tokyo) 1989, 42: 564–570.CrossRefGoogle Scholar
  3. [3]
    Tamaoki T, Nomoto H, Takahashi I, Kato Y, Morimoto M, Tomita F. Staurosporine, a potent inhibitor of phospholipid/Ca++dependent protein kinase. Biochem Biophys Res Commun 1986, 135: 397–402.PubMedCrossRefGoogle Scholar
  4. [4]
    Gani OA, Engh RA. Protein kinase inhibition of clinically important staurosporine analogues. Nat Prod Rep 2010, 27: 489–498.PubMedCrossRefGoogle Scholar
  5. [5]
    Ringman JM, Frautschy SA, Cole GM, Masterman DL, Cummings JL. A potential role of the curry spice curcumin in Alzheimer’s disease. Curr Alzheimer Res 2005, 2: 131–136.PubMedCrossRefGoogle Scholar
  6. [6]
    Kim DS, Park SY, Kim JK. Curcuminoids from Curcuma longa L. (Zingiberaceae) that protect PC12 rat pheochromocytoma and normal human umbilical vein endothelial cells from betaA(1-42) insult. Neurosci Lett 2001, 303: 57–61.PubMedCrossRefGoogle Scholar
  7. [7]
    Xu YX, Pindolia KR, Janakiraman N, Chapman RA, Gautam SC. Curcumin inhibits IL1 alpha and TNF-alpha induction of AP-1 and NF-kB DNA-binding activity in bone marrow stromal cells. Hematopathol Mol Hematol 1997, 11: 49–62.PubMedGoogle Scholar
  8. [8]
    Pan MH, Lin-Shiau SY, Lin JK. Comparative studies on the suppression of nitric oxide synthase by curcumin and its hydrogenated metabolites through down-regulation of IkappaB kinase and NFkappaB activation in macrophages. Biochem Pharmacol 2000, 60: 1665–1676.PubMedCrossRefGoogle Scholar
  9. [9]
    Ono K, Hasegawa K, Naiki H, Yamada M. Curcumin has potent anti-amyloidogenic effects for Alzheimer’s beta-amyloid fibrils in vitro. J Neurosci Res 2004, 75: 742–750.PubMedCrossRefGoogle Scholar
  10. [10]
    Qin XY, Cheng Y, Cui J, Zhang Y, Yu LC. Potential protection of curcumin against amyloid beta-induced toxicity on cultured rat prefrontal cortical neurons. Neurosci Lett 2009, 463: 158–161.PubMedCrossRefGoogle Scholar
  11. [11]
    Calabrese V, Scapagnini G, Colombrita C, Ravagna A, Pennisi G, Giuffrida Stella AM, et al. Redox regulation of heat shock protein expression in aging and neurodegenerative disorders associated with oxidative stress: a nutritional approach. Amino Acids 2003, 25: 437–444.PubMedCrossRefGoogle Scholar
  12. [12]
    Papasozomenos S, Shanavas A. Testosterone prevents the heat shock-induced overactivation of glycogen synthase kinase-3 beta but not of cyclin-dependent kinase 5 and c-Jun NH2-terminal kinase and concomitantly abolishes hyperphosphorylation of tau: implications for Alzheimer’s disease. Proc Natl Acad Sci U S A 2002, 99: 1140–1145.PubMedCrossRefGoogle Scholar
  13. [13]
    Setalo G Jr, Singh M, Guan X, Toran-Allerand CD. Estradiolinduced phosphorylation of ERK1/2 in explants of the mouse cerebral cortex: the roles of heat shock protein 90 (Hsp90) and MEK2. J Neurobiol 2002, 50: 1–12.PubMedCrossRefGoogle Scholar
  14. [14]
    Zamostiano R, Pinhasov A, Bassan M, Perl O, Steingart RA, Atlas R, et al. A femtomolar-acting neuroprotective peptide induces increased levels of heat shock protein 60 in rat cortical neurons: a potential neuroprotective mechanism. Neurosci Lett 1999, 264: 9–12.PubMedCrossRefGoogle Scholar
  15. [15]
    Hill MM, Hemmings BA. Inhibition of protein kinase B/Akt. Implications for cancer therapy. Pharmacol Ther 2002; 93: 243–251.PubMedCrossRefGoogle Scholar
  16. [16]
    Burugula B, Ganesh BS, Chintala SK. Curcumin attenuates staurosporine-mediated death of retinal ganglion cells. Invest Ophthalmol Vis Sci 2011, 52: 4263–4273.PubMedCrossRefGoogle Scholar
  17. [17]
    Sharma RA, Gescher AJ, Steward WP. Curcumin: the story so far. Eur J Cancer 2005, 41: 1955–1968.PubMedCrossRefGoogle Scholar
  18. [18]
    Chiu SS, Lui E, Majeed M, Vishwanatha JK, Ranjan AP, Maitra A, et al. Differential distribution of intravenous curcumin formulations in the rat brain. Anticancer Res 2011, 31: 907–911.PubMedGoogle Scholar
  19. [19]
    Frautschy SA, Hu W, Kim P, Miller SA, Chu T, Harris-White ME et al. Phenolic anti-inflammatory antioxidant reversal of Abetainduced cognitive deficits and neuropathology. Neurobiol Aging 2001, 22: 993–1005.PubMedCrossRefGoogle Scholar
  20. [20]
    Li WP, Chan WY, Lai HW, Yew DT. Terminal dUTP nick end labeling (TUNEL) positive cells in the different regions of the brain in normal aging and Alzheimer patients. J Mol Neurosci 1997, 8: 75–82.PubMedCrossRefGoogle Scholar
  21. [21]
    Gomez-Isla T, Hollister R, West H, Mui S, Growdon JH, Petersen RC, et al. Neuronal loss correlates with but exceeds neurofibrillary tangles in Alzheimer’s disease. Ann Neurol 1997, 41: 17–24.PubMedCrossRefGoogle Scholar

Copyright information

© Shanghai Institutes for Biological Sciences, CAS and Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Xiao-Yan Qin
    • 1
    • 2
  • Ji-Hui Lv
    • 3
  • Jia Cui
    • 1
    • 2
  • Xue Fang
    • 1
    • 2
  • Yan Zhang
    • 1
    • 2
    Email author
  1. 1.State Key Laboratory of Biomembrane and Membrane Biotechnolog, College of Life SciencesPeking UniversityBeijingChina
  2. 2.Laboratory of Biotechnology and State Key Laboratory of Chinese Ethnic Minority Traditional Medicine, College of Life & Environmental ScienceMinzu University of ChinaBeijingChina
  3. 3.Beijing Geriatric HospitalBeijingChina

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