Sevoflurane exposure in 7-day-old rats affects neurogenesis, neurodegeneration and neurocognitive function
- 454 Downloads
Sevoflurane is widely used in pediatric anesthesia and former studies showed that it causes neurodegeneration in the developing brain. The present study was carried out to investigate the effects of sevoflurane on neurogenesis, neurodegeneration and behavior.
We administered 5-bromodeoxyuridine, an S-phase marker, before, during, and after 4 h of sevoflurane given to rats on postnatal day 7 to assess dentate gyrus progenitor proliferation and Fluoro-Jade staining for degeneration. Spatial reference memory was tested 2 and 6 weeks after anesthesia.
Sevo-flurane decreased progenitor proliferation and increased cell death until at least 4 days after anesthesia. Spatial reference memory was not affected at 2 weeks but was affected at 6 weeks after sevoflurane administration.
Sevoflurane reduces neurogenesis and increases the death of progenitor cells in developing brain. This might mediate the late-onset neurocognitive outcome after sevoflurane application.
Keywordssevoflurane neurogenesis progenitor proliferation degeneration
Unable to display preview. Download preview PDF.
- Fredriksson A, Ponten E, Gordh T, Eriksson P. Neonatal exposure to a combination of N-methyl-D-aspartate and gamma-aminobutyric acid type A receptor anesthetic agents potentiates apoptotic neurodegeneration and persistent behavioral deficits. Anesthesiology 2007, 107: 427–436.PubMedCrossRefGoogle Scholar
- Schmued LC, Albertson C, Slikker W Jr. Fluoro-Jade: a novel fluorochrome for the sensitive and reliable histochemical localization of neuronal degeneration. Brain Res Brain Res Rev 1997, 751: 37–46.Google Scholar