Neuroscience Bulletin

, Volume 28, Issue 5, pp 499–508 | Cite as

Sevoflurane exposure in 7-day-old rats affects neurogenesis, neurodegeneration and neurocognitive function

  • Fang Fang
  • Zhanggang Xue
  • Jing CangEmail author
Original Article



Sevoflurane is widely used in pediatric anesthesia and former studies showed that it causes neurodegeneration in the developing brain. The present study was carried out to investigate the effects of sevoflurane on neurogenesis, neurodegeneration and behavior.


We administered 5-bromodeoxyuridine, an S-phase marker, before, during, and after 4 h of sevoflurane given to rats on postnatal day 7 to assess dentate gyrus progenitor proliferation and Fluoro-Jade staining for degeneration. Spatial reference memory was tested 2 and 6 weeks after anesthesia.


Sevo-flurane decreased progenitor proliferation and increased cell death until at least 4 days after anesthesia. Spatial reference memory was not affected at 2 weeks but was affected at 6 weeks after sevoflurane administration.


Sevoflurane reduces neurogenesis and increases the death of progenitor cells in developing brain. This might mediate the late-onset neurocognitive outcome after sevoflurane application.


sevoflurane neurogenesis progenitor proliferation degeneration 


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Copyright information

© Shanghai Institutes for Biological Sciences, CAS and Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  1. 1.Department of Anesthesiology, Zhongshan HospitalFudan UniversityShanghaiChina

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