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Neuroscience Bulletin

, Volume 28, Issue 5, pp 532–540 | Cite as

Schizandrin prevents dexamethasone-induced cognitive deficits

  • Xiao Xu
  • Xing Zhou
  • Xiao-Wen Zhou
  • Zheng Zhang
  • Min-Jing Liao
  • Qi Gao
  • Huan-Min LuoEmail author
Original Article

Abstract

Objective

To model glucocorticoid-induced cognitive impairment and evaluate the neuroprotection by schizandrin (Sch) against dexamethasone (Dex)-induced neurotoxicity in vivo and in vitro.

Methods

Cerebral cortical cells from neonatal Sprague-Dawley rats (within 24 hours after birth) were cultured for 9 days, and then treated with Dex (10−4, 10−5, 10−6 or 10−7 mol/L) for 24 h or pretreated with 10−4 mol/L Dex for 24 h followed by 10, 20, 40, or 80 μmol/L Sch for 48 h. Cell viability was assessed using the MTT assay. Immunofluorescence and real-time PCR for MAP2 were performed to confirm the effects of Dex on neurite outgrowth. In vivo, kunming mice were randomly divided into six groups: control [(intragastric (i.g.) vehicle for 42 days]; Dex group I (5 mg/kg·d−1 Dex i.g. treatment for 28 days followed by i.g. vehicle for 14 days); Dex group II (Dex i.g. for 42 days); Dex + Sch (Dex i.g. for 28 days followed by 5, 15, or 45 mg/kg·d−1 Sch i.g. for 14 days). Learning and memory were assessed by Morris water maze test. Histological examination was used to assess pathology and apoptosis in neurons.

Results

Compared to the Dex groups, Sch increased cell viability in a dosedependent manner, improved performance in the Morris water maze and ameliorated the morphological changes.

Conclusion

Sch has neuroprotective effects against insults induced by glucocorticoid.

Keywords

neuron glucocorticoid cognitive impairment 

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Supplementary material

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Copyright information

© Shanghai Institutes for Biological Sciences, CAS and Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Xiao Xu
    • 1
    • 2
  • Xing Zhou
    • 1
  • Xiao-Wen Zhou
    • 1
  • Zheng Zhang
    • 1
  • Min-Jing Liao
    • 1
  • Qi Gao
    • 3
  • Huan-Min Luo
    • 1
    • 3
    Email author
  1. 1.Department of Pharmacology, School of MedicineJinan UniversityGuangzhouChina
  2. 2.Department of Physiology, School of MedicineHenan UniversityKaifengChina
  3. 3.The Joint Laboratory of Brain Function and Health, Jinan University and the University of Hong KongJinan UniversityGuangzhouChina

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