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Neuroscience Bulletin

, Volume 28, Issue 4, pp 351–374 | Cite as

Emerging approaches to probing ion channel structure and function

  • Wei-Guang Li
  • Tian-Le XuEmail author
Review

Abstract

Ion channels, as membrane proteins, are the sensors of the cell. They act as the first line of communication with the world beyond the plasma membrane and transduce changes in the external and internal environments into unique electrical signals to shape the responses of excitable cells. Because of their importance in cellular communication, ion channels have been intensively studied at the structural and functional levels. Here, we summarize the diverse approaches, including molecular and cellular, chemical, optical, biophysical, and computational, used to probe the structural and functional rearrangements that occur during channel activation (or sensitization), inactivation (or desensitization), and various forms of modulation. The emerging insights into the structure and function of ion channels by multidisciplinary approaches allow the development of new pharmacotherapies as well as new tools useful in controlling cellular activity.

Keywords

ion channel structure and function mutagenesis covalent modification voltage-clamp fluorometry computational chemistry 

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Copyright information

© Shanghai Institutes for Biological Sciences, CAS and Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  1. 1.Neuroscience Division, Department of Biochemistry and Molecular Cell Biology, Institute of Medical SciencesShanghai Jiao Tong University School of MedicineShanghaiChina

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