Neuroscience Bulletin

, Volume 27, Issue 3, pp 135–142 | Cite as

Effects of SKF-96365, a TRPC inhibitor, on melittin-induced inward current and intracellular Ca2+ rise in primary sensory cells

  • Jing Ding (丁静)
  • Yong Xiao (肖勇)
  • Dan Lu (吕丹)
  • Yi-Ru Du (杜意如)
  • Xiu-Yu Cui (崔秀玉)
  • Jun Chen (陈军)Email author
Original Article



Melittin (MEL) is a major component of bee venom and can produce both persistent spontaneous nociception and pain hypersensitivity when injected subcutaneously in the periphery. The present study aimed to examine the roles of transient receptor potential canonical (TRPC) channels in mediation of MEL-induced activation of primary nociceptive cells.


Whole-cell patch-clamp and laser scanning confocal calcium detection were used to evaluate the effects of SKF-96365, a TRPC inhibitor, applied on the acutely isolated dorsal root ganglion (DRG) cells of rat, on MEL-induced increase in intracellular calcium concentration ([Ca2+]i) and inward current.


Under voltageclamp mode, 43.9% (40/91) DRG cells were evoked to give rise to the inward current by 2 μmol/L MEL, which could be significantly suppressed by 3 doses of SKF-96365 (1, 5 and 10 μmol/L) in a dose-dependent manner. Of the other 210 cells, 67.6% responded to MEL with an intracellular Ca2+ rise, as revealed by confocal calcium imaging. Of these MEL-sensitive cells, 46.5% (66/142) were suppressed by the highest dose of SKF-96365.


MEL-induced activation of small to medium-sized DRG cells can be suppressed by SKF-96365, suggesting the involvement of TRPC channels in the mediation of MEL-induced activation of primary nociceptive cells.


melittin transient receptor potential canonical channel dorsal root ganglion patch-clamp technique calcium imaging 

TRPC 通道阻断剂SKF-96365 对蜜蜂毒肽诱发初级感觉细胞内向电流和胞内钙增高的作用



蜜蜂毒肽是蜜蜂粗毒中的主要物质。 外周皮下注射蜜蜂毒肽可导致持续性自发痛和痛觉过敏。 本研究旨在研究瞬时受体电势C (transient receptor potential canonical, TRPC) 通道在蜜蜂毒肽诱致的初级感觉神经元活化中的介导作用。


运用全细胞膜片钳和激光共聚焦测钙技术, 检测TRPC通道抑制剂SKF-96365对蜜蜂毒肽诱致的急性分离大鼠背根神经节细胞胞内钙和内向电流升高的影响。


电压钳记录的91个背根神经节细胞中, 蜜蜂毒肽可诱发43.9% (40/91)的细胞产生内向电流, 而不同浓度的SKF-96365 (1, 5, 10 μmol/L) 均明显抑制了背根神经节细胞的内向电流, 且呈剂量相关性。 应用激光共聚焦钙成像技术记录的210个背根神经节细胞中, 67.6% 的细胞对蜜蜂毒肽敏感, 产生胞内钙离子浓度的升高, 而SKF-96365能抑制这种胞内钙浓度的升高, 抑制率为46.5%。


SKF-96365能够抑制蜜蜂毒肽引起的背根神经节中小神经元的活化, 提示TRPC通道介导了蜜蜂毒肽对初级感觉神经元的激活作用。


蜜蜂毒肽 瞬时受体电势C通道 背根神经节 膜片钳技术 激光共聚焦测钙 


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Copyright information

© Shanghai Institutes for Biological Sciences, CAS and Springer-Verlag Berlin Heidelberg 2011

Authors and Affiliations

  • Jing Ding (丁静)
    • 1
  • Yong Xiao (肖勇)
    • 2
  • Dan Lu (吕丹)
    • 1
  • Yi-Ru Du (杜意如)
    • 1
  • Xiu-Yu Cui (崔秀玉)
    • 1
  • Jun Chen (陈军)
    • 1
    • 2
    Email author
  1. 1.Institute for Biomedical Sciences of PainCapital Medical UniversityBeijingChina
  2. 2.Institute for Biomedical Sciences of Pain and Institute for Functional Brain Disorders, Tangdu Hospitalthe Fourth Military Medical UniversityXi’anChina

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