Genes & Nutrition

, 9:410 | Cite as

Multivitamin restriction increases adiposity and disrupts glucose homeostasis in mice

  • Nisserine Ben Amara
  • Julie Marcotorchino
  • Franck Tourniaire
  • Julien Astier
  • Marie-Josèphe Amiot
  • Patrice Darmon
  • Jean-François Landrier
Research Paper

Abstract

A strong association between obesity and low plasma concentrations of vitamins has been widely reported; however, the causality of this relationship is still not established. Our goal was to evaluate the impact of a multivitamin restriction diet (MRD) on body weight, adiposity and glucose homeostasis in mice. The mice were given a standard diet or a diet containing 50 % of the recommended vitamin intake (MRD) for 12 weeks. At the end of the experiment, total body weight was 6 % higher in MRD animals than in the control group, and the adiposity of the MRD animals more than doubled. The HOMA-IR index of the MRD animals was significantly increased. The adipose tissue of MRD animals had lower expression of mRNA encoding adiponectin and Pnpla2 (47 and 32 %, respectively) and 43 % higher leptin mRNA levels. In the liver, the mRNA levels of Pparα and Pgc1α were reduced (29 and 69 %, respectively) in MRD mice. Finally, the level of β-hydroxybutyrate, a ketonic body reflecting fatty acid oxidation, was decreased by 45 % in MRD mice. Our results suggest that MRD promotes adiposity, possibly by decreasing adipose tissue lipolysis and hepatic β-oxidation. These results could highlight a possible role of vitamin deficiency in the etiology of obesity and associated disorders.

Keywords

Vitamins Obesity Adiposity HOMA-IR Healthy pattern 

Supplementary material

12263_2014_410_MOESM1_ESM.docx (12 kb)
Supplementary material 1 (DOCX 11 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Nisserine Ben Amara
    • 1
    • 2
    • 3
  • Julie Marcotorchino
    • 1
    • 2
    • 3
  • Franck Tourniaire
    • 1
    • 2
    • 3
  • Julien Astier
    • 1
    • 2
    • 3
  • Marie-Josèphe Amiot
    • 1
    • 2
    • 3
  • Patrice Darmon
    • 1
    • 2
    • 3
  • Jean-François Landrier
    • 1
    • 2
    • 3
  1. 1.INRA, UMR 1260MarseilleFrance
  2. 2.INSERM, UMR 1062Nutrition, Obésité et Risque ThrombotiqueMarseilleFrance
  3. 3.UMR 1260 INRA/1062 INSERM, Faculté de MédecineAix-Marseille UniversitéMarseille Cedex 05France

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