Genes & Nutrition

, Volume 4, Issue 1, pp 59–67

Feeding a ROS-generator to Caenorhabditis elegans leads to increased expression of small heat shock protein HSP-16.2 and hormesis

  • Kai Hartwig
  • Tanja Heidler
  • Jan Moch
  • Hannelore Daniel
  • Uwe Wenzel
Research Paper

DOI: 10.1007/s12263-009-0113-x

Cite this article as:
Hartwig, K., Heidler, T., Moch, J. et al. Genes Nutr (2009) 4: 59. doi:10.1007/s12263-009-0113-x

Abstract

Reactive oxygen species (ROS) are thought to be a driving force in the aging process. In transgenic Caenorhabditis elegans expressing green fluorescent protein (GFP) under control of the hsp-16.2 promoter (CL2070) 100 μM of the ROS-generator juglone induced GFP-expression. This was associated with translocation of DAF-16 to the nucleus as visualized in a transgenic strain expressing a DAF-16::GFP fusion protein (TJ356) and with increased cellular levels of reduced glutathione. RNA-interference for DAF-16 in CL2070 blocked the juglone-induced HSP-16.2 expression and the increase in glutathione levels. Higher concentrations of juglone did not further increase the adaptive responses but caused premature death, indicating hormetic adaptations unless the stressor exceeds the intrinsic protective capacity. The addition of the ROS-scavenger ascorbic acid finally blocked lifespan reductions and all of the adaptations to juglone stressing that ROS are indeed the molecular species that require protective response.

Keywords

Caenorhabditis elegans Hormesis Reactive oxygen species Aging Heat shock protein Glutathione 

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Kai Hartwig
    • 1
  • Tanja Heidler
    • 1
  • Jan Moch
    • 2
  • Hannelore Daniel
    • 1
  • Uwe Wenzel
    • 2
  1. 1.Department of Food and Nutrition, Molecular Nutrition UnitTechnical University of MunichFreisingGermany
  2. 2.Molecular Nutrition Research, Interdisciplinary Research CenterJustus-Liebig-University of GiessenGiessenGermany

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