A mixture of the aqueous extract of Garcinia cambogia, soy peptide and l-carnitine reduces the accumulation of visceral fat mass in rats rendered obese by a high fat diet
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The aim of the present study was to investigate the anti-obesity effect of a mixture composed of Garcinia cambogia extract, soypeptide, and l-carnitine (1.2:0.3:0.02, w/w/w) in rats rendered obese by a high-fat diet (HFD). Sprague-Dawley rats were fed either the high-fat control diet (CD) or the 0.38% mixture-supplemented HFD (CD + M) for 9 weeks. The mixture significantly reduced body weight gain and the accumulation of visceral fat mass in a rat model of HFD-induced obesity. Moreover, the mixture effectively lowered blood and hepatic lipid concentrations and serum glucose, insulin, c-peptide, and leptin levels in rats with HFD-induced obesity. Results from real-time reverse transcription-polymerase chain reaction analyses indicated that the expression levels of leptin, tumor necrosis factor-alpha (TNF-α), and sterol regulatory element binding protein 1c (SREBP1c) genes in the epididymal fat tissue of rats fed the CD + M diet were 0.4-, 0.6-, and 0.48-fold, respectively, of those found in the CD rats (P < 0.05), while expression of the uncoupling protein 2 (UCP2) gene in epididymal adipose tissue was 1.25-fold (P < 0.05) of that found in CD rats. In conclusion, a mixture composed of G. cambogia extract, soy peptide, and l-carnitine attenuated visceral fat accumulation and improved dyslipidemia in a rat model with HFD-induced obesity.
KeywordsGarcinia cambogia Soypeptide l-carnitine Anti-obesity effect High-fat-diet-induced obesity rats Obesity-related genes
Obesity is one of the most serious and the fastest growing public health problems throughout the industrialized world. Obesity, especially with visceral fat accumulation, is a serious risk factor for so-called metabolic syndrome, which includes insulin resistance, glucose intolerance, hypertension, and dyslipidemia . The recent epidemic increase in obesity in developed countries points to the important interaction between genes that predispose to obesity and environmental factors that facilitate expression of the obese phenotype, a trait shared with high-fat diet (HFD)-induced obesity rodent models .
Studies on obesity in the field of food science have focused on the search for functional food ingredients and/or herbal extracts that can suppress the accumulation of body fat. Some herbal products and plant extracts, such as Semen Cassiaem , Panax ginseng berry extract , Singiber officinale Roscoe , and Platycodi radix , have been shown to exert anti-obesity effects in rodents with HFD-induced obesity. Anti-obesity food ingredients and herbal extracts may also prevent lifestyle-related diseases, if they are effective in reducing body fat accumulation.
Garcinia cambogia, an edible fruit native to southeastern Asia, contains large quantities of hydroxy citric acid (HCA), which has been shown to inhibit ATP citrate lyase (EC 22.214.171.124) , suppress de novo fatty acid synthesis and food intake, and consequently decrease body weight gain . Several studies in animals and humans have shown that consumption of soybean has beneficial effects in a variety of disorders including hypocholesterolemia, cardiovascular disease, renal disease, bone resorption, certain forms of cancer, and obesity [2, 7, 37]. Soy protein or peptide has been thought to be responsible for the cholesterol-lowering effect of soybean [3, 13]. l-Carnitine (β-hydroxy-γ-trimethylaminobutyric acid) is a small, water-soluble, quaternary amine that is responsible for maintaining the energy metabolism in mammals as an essential cofactor in the transport of long-chain fatty acids across the inner mitochondrial membrane for subsequent fat degradation and energy production .
The present study was conducted to test our hypothesis that metabolic changes induced by a mixture of G. cambogia, soy peptide, and l-carnitine are associated with a reduction in HFD-induced adiposity, especially visceral fat mass, in rats. To provide a possible scientific basis for the extensive usage of these three functional food ingredients, we investigated their effects as a mixture on visceral fat mass, lipid profiles in the serum and liver, and serum adipocytokine levels. Mixture-induced regulation of the expression of multiple adipose tissue genes was evaluated in rats rendered obese by a high-fat diet.
Materials and methods
Animals and diets
Five-week-old male Sprague-Dawley rats (n = 20, Orient Co, Seoul, Korea) were individually housed in stainless steel rat cages in a room where the temperature was kept at 21 ± 2.0°C, the relative humidity was kept at 50 ± 5%, and the light was maintained on a 12 h light/dark cycle. All the rats consumed a commercial diet and tap water ad libitum for 1 week prior to their allocation to one of two weight-matched groups: the high-fat control diet group (CD) and the mixture-supplemented HFD group (CD + M). The CD was based on the AIN-76 rodent diet composition and contained 200 g fat/kg (170 g lard plus 30 g corn oil) and 1% cholesterol by weight. The HFD was formulated to provide 40% of the total energy generated by the diet from fat, by replacing carbohydrate energy with lard and corn oil, and had the same amount of vitamins and minerals per kilojoule as the normal diet. The CD + M was identical to the CD but additionally contained 0.38% mixture composed of G. cambogia extract (InterHealth Co, Benicia, CA; containing 60% HCA), soy peptide (Fuji Oil Co, Ibaraki, Japan) and l-carnitine (Lonza, Basel, Switzerland) in the proportion of 1.2:0.3:0.02 (w/w/w). The diets were given in the form of pellets for 9 weeks.
This study adhered to the Guide for the Care and Use of Laboratory Animals developed by the Institute of Laboratory Animal Resources of the National Research Council, and approved by the Institutional Animal Care and Use Committee of Yonsei University in Seoul, South Korea.
Serum concentrations of total cholesterol, HDL cholesterol, triglyceride (Young-dong Diagnostics, Seoul, Korea), and free fatty acid (Eiken Chemical, Tokyo, Japan) were determined enzymatically using commercial kits. The serum VLDL + LDL cholesterol concentration was calculated by subtracting the concentration of HDL cholesterol from the total cholesterol concentration.
Hepatic lipids were extracted as described previously , and the dried lipid residues were dissolved in 1 ml ethanol. The concentrations of cholesterol and triglycerides in hepatic lipid extracts were measured using the same enzymatic kits used for the serum lipid analyses.
Serum insulin, adipocytokines and glucose assays
Serum insulin, c-peptide, and leptin levels were measured by radioimmunoassay (RIA rat insulin, rat c-peptide, and rat leptin kits; Linco Research, St. Charles, MO). The serum glucose concentration was determined using an automatic analyzer (Express Plus, Chiron Diagnostics, Emeryville, CA) with reagents from Bayer (Leverkusen, Germany).
Total RNA was isolated from the epididymal fat tissues of each rat using Trizol (Invitrogen, Carlsbad, CA), according to the manufacturer’s instructions. The quantity and quality of the RNA samples was assessed using a Optima TLX-120 spectrophotometer (Beckman, Fullerton, CA) and an Agilent 2100 bioanalyzer (Agilent Technologies, Wilmington, DE).
Primers for real-time PCR analysis were designed using the Whitehead Institute/MT Center for Genome Research’s Primer3 interface, which is available online. The sequences of the designed primers were as follows: leptin- sense-5′-CACAGAGGTGGTGGCTCTGA-3′ and antisense-5′-CCCGGTGGTCTTGGAAACTT-3′; tumor necrosis factor α (TNFα)-sense-5′-AGATCATCTTCTCAAAACTC-3′ and antisense-5′-TAAGTACTTGGGCAGGTTGA-3′; resistin- sense-5′-ACTTCAGCTCCCTACTGCCA-3′ and antisense-5′-GCTCAGTTCTCATCAATCAACCGTCC-3′; sterol-regulatory-element-binding protein-1c (SREBP1c)- sense-5′-GGAGCCATGGATTGCACATT-3′ and antisense-5′-AGGAAGGCTTCCAGAGAGGA-3′; peroxisome proliferators’ activated receptor γ2 (PPARγ2)- sense-5′-CTTGGCCATATTTATAGCTGTCATTATT-3′ and antisense-5′-TGTCCTCGATGGGCTTCAC-3′; CCAAT/enhancer-binding protein α (C/EBPα)- sense-5′-GGCGGGAACGCAACAA-3′ and antisense-5’-TCCACGTTGCGCTGTTTG-3′; uncoupling protein 2 (UCP2)- sense-5′-ACAAGACCATTGCACGAGAG-3′ and antisense-5′-CATGGTCAGGGCACAGTGGC-3′; and β-actin- sense-5′-ACCTTCAACACCCCAGCCATGTACG-3′ and antisense-5′-CTGATCCACATCTGCTGGAAGGTGG-3′.
Total RNA (1 μg) was reverse-transcribed using a Superscript II kit (Invitrogen), according to the manufacturer’s recommendations. Real-time PCR reactions were then carried out in a 20 μl reaction mixture (2 μl cDNA; 16 μl SYBR Green PCR Master Mix, which includes 2 μl 1 × LightCycler; 2.4 μl 1.5 mM MgCl2 and 11.6 μl H2O; and 1 μl of a 0.5 μmol/l specific gene primer pair solution) in a LightCycler (Roche Diagnostics, Basel, Switzerland). The PCR program was initiated with a 10 min reaction at 95°C before 40 thermal cycles of 10 sec each at 95°C, 5 sec at 55°C, and 30 sec at 70°C, were conducted. The data obtained were analyzed using the comparative-cycle threshold method, and were normalized using the β-actin expression value. Melting curves were generated for each PCR reaction to ensure the purity of the amplification product.
The data are presented as mean ± SEM. Two-tailed Student’s t tests were used to identify the significant differences (P < 0.05) between the means for CD and CD + M rats.
Body weight gain and visceral fat-pad weights
Body weight gain, visceral fat-pad weight, and serum and hepatic biochemistry of rats fed the high-fat control diet (CD) or the mixture-supplemented diet (CD + M) for 9 weeks. Values are mean ± SEM, n = 10
CD + M
Body weight gain (g/9 weeks)
463 ± 23.3
385 ± 15.4*
Food efficiency ratioa
0.27 ± 0.009
0.24 ± 0.007*
Visceral fat-pad weight (g/100 g body weight)
2.86 ± 0.27
2.35 ± 0.10
0.85 ± 0.05
0.57 ± 0.04**
3.33 ± 0.22
2.79 ± 0.05*
2.29 ± 0.10
1.94 ± 0.03*
9.33 ± 0.58
7.65 ± 0.15*
Total cholesterol (mmol/l)
4.43 ± 0.58
2.63 ± 0.24*
HDL cholesterol (mmol/l)
1.21 ± 0.09
1.03 ± 0.06
VLDL + LDL cholesterolb (mmol/l)
2.55 ± 0.32
1.60 ± 0.21*
0.37 ± 0.04
0.32 ± 0.04
Free fatty acid (mmol/l)
0.60 ± 0.04
0.35 ± 0.02***
10.4 ± 0.58
8.07 ± 0.44**
641 ± 14.4
178 ± 44.5*
2,047 ± 226
832 ± 134**
11.0 ± 1.57
6.09 ± 0.84*
Liver weight (g/100 g of body weight)
5.01 ± 0.18
4.36 ± 0.09**
Cholesterol (μmol/g of liver)
16.4 ± 0.42
14.9 ± 0.32*
Triglyceride (μmol/g of liver)
17.7 ± 0.31
16.6 ± 0.30*
The relative weights of the visceral fat deposits were smaller in the CD + M rats than in the CD rats (Table 1). The epididymal, perirenal, retroperitoneal, and mesenteric fat-pad weights were reduced by 18% (P > 0.05), 33% (P < 0.01), 16% (P < 0.05), and 15% (P < 0.05), respectively, in rats that had been fed the CD + M compared to the CD rats. The mixture-induced reduction in the accumulation of visceral fat mass was most significant in the perirenal fat tissue compared with visceral fat tissues located elsewhere. The total weight of visceral fat deposits pooled from the four different locations was 18% lower in the CD + M rats than in the CD rats (P < 0.05).
Blood and hepatic biochemistry
The serum total and LDL + VLDL cholesterol concentrations were 41 and 37% lower, respectively, in rats fed the CD + M compared to those of the CD rats (P < 0.05) (Table 1). Dietary supplementation of the mixture to rats fed the HFD significantly decreased the serum free fatty acid concentration (42% reduction, P < 0.001) without significantly altering the serum triglyceride concentration. Dietary supplementation of the mixture for 9 weeks also significantly reduced the hepatic cholesterol and triglyceride concentrations in rats fed the HFD. The relative weight of the liver was significantly smaller in the CD + M rats than in the CD rats. Moreover, dietary supplementation of the mixture to rats fed the HFD resulted in significant decreases in the serum concentrations of glucose (22% reduction, P < 0.01), insulin (72% reduction, P < 0.05), c-peptide (59% reduction, P < 0.01) and leptin (45% reduction, P < 0.05), as summarized in Table 1.
Gene expression profiles in the epididymal adipose tissue
We have previously observed that a HFD (40% fat calories), with identical composition to the high-fat CD used in the current study, produces obese conditions in rats that mimic human obesity. HFD-induced obesity rats weigh 55% more and accumulate 85–133% more visceral fats, depending on the location, than normal rats. These obese rats acquire dyslipidemia, fatty liver, insulin resistance, and hyperleptinemia along with over-expression of leptin, TNF-α, resistin, PPARγ2, C/EBPα, and SREBP1c genes in epididymal adipose tissue (manuscript submitted). An anti-obesity effect of a mixture composed of the G. cambogia extract, soy peptide and l-carnitine was observed in the HFD-induced obesity rat model. The results from the present study clearly demonstrate that the mixture significantly reduces the accumulation of visceral fat mass and effectively lowers blood and hepatic lipid levels, leading to the improvement of insulin resistance in rats rendered obese by HFD.
The mixture of G. cambogia, soy peptide, and l-carnitine appears to exert its anti-obesity effect via modulation of the metabolic derangement induced by the HFD, and might involve interactions between multiple genes implicated in the process of visceral adiposity and the dietary intervention, rather than by simply suppressing appetite. As supplementation of the mixture to the HFD did not affect the food intake of the animals (data not shown), it is unlikely that the anti-obesity effect of the mixture results from a refusal to ingest the food.
Decreases in the levels of serum and hepatic lipids, such as total cholesterol, VLDL + LDL cholesterol, and free fatty acid, in rats fed CD + M compared to those for CD rats could be attributed to the inhibition of lipid absorption in the gastrointestinal tract. Dietary lipids are absorbed into the bloodstream as chylomicron; triglycerides in these chylomicrons are then digested as fatty acids and glycerol by lipoprotein lipase, and are eventually transported and stored in the liver and adipose tissues in the form of triglycerides. The remnants of the chylomicrons are taken up mainly by the liver, and are then transformed into lipoproteins, such as VLDL, which transport triglycerides synthesized in the liver to adipose tissues, and LDL, which transports cholesterol to peripheral tissues .
The mixture-induced decreases in serum glucose, insulin, and c-peptide levels of rats fed the HFD may account for the improvement in insulin resistance. Among the various body fat deposits, the visceral fat mass is best correlated to insulin resistance in animal models and humans . Insulin action is markedly impaired in individuals with visceral obesity , and the removal of visceral fat mass prevents the insulin resistance and glucose intolerance associated with aging . Two groups of inflammatory proteins are produced and released by adipose tissue: (1) inflammatory mediators, predominantly IL-6 and TNF-α produced by adipose tissue and macrophages, and (2) adipocytokines such as leptin, adiponectin and resistin . Leptin is a fat-derived key regulator of appetite and energy expenditure, and serum leptin concentration is associated with general adiposity . The slight reduction in the fasting blood glucose level (22% lower) in spite of the marked decrease in serum insulin level (72% lower) in the CD + M rats compared to levels in CD rats indicates improved insulin sensitivity in rats fed the mixture. The mixture-induced amelioration of insulin resistance is supported by the down-regulation of leptin and TNFα gene expression in the epididymal adipose tissues of rats fed the mixture (Fig. 1).
Adipocyte growth and differentiation are complex processes that are characterized by many changes in cell morphology, hormone sensitivity, and expression of genes controlling lipogenesis and lipolysis . Several transcription factors, such as members of the PPARγ2, C/EBPs, and SREBP1c family, act cooperatively and sequentially to trigger the terminal adipocyte differentiation program [9, 11]. PPARγ is an adipocyte-specific transcription factor that appears to promote adipocyte differentiation and to control the expression of several fat-specific genes . The C/EBP proteins that are also important in adipogenesis are expressed at high levels in adipose tissues and are induced during adipogenesis . C/EBPα, in powerful synergy with PPARγ2, promotes the terminal differentiation of preadipocytes . SREBP1c controls the production of endogenous ligands for PPARγ as a mechanism for coordinating the actions of these adipogenic factors . The down-regulation of SREBP1c and PPARγ2 gene expression in the epididymal fat tissues of rats given CD + M may explain the mixture-induced regulation of adipocyte metabolism and its differentiation process. In brown and white adipose tissues, the properties of UCP2 appear to be suited to the regulation of fuel metabolism . Most animal models show up-regulation of UCP2 and/or UCP3 by HFDs, although this has not been universally observed. Up-regulation of UCP expression depends on the strain and tissue type. A high fat diet increased UCP3 mRNA expression in the skeletal muscle of C57BL/6J mice  and rats  but increased UCP2 expression only slightly in white adipose tissue of AKR mice and not at all in C57BL/6J mice  or in rats .
Hydroxy citric acid is a potent competitive inhibitor of ATP citrate lyase (EC 126.96.36.199) , which is an extra-mitochondrial enzyme catalyzing the cleavage of citrate to oxaloacetate and acetyl-CoA. This inhibitory action of HCA reduces the acetyl-CoA pool, thus limiting the availability of the two-carbon units required for the initial steps of fatty acid and cholesterol biosynthesis [6, 34]. The reduction in the acetyl-CoA pool is thought to decrease the concentration of malonyl-CoA, thus resulting in the suppression of body fat accumulation through stimulation of carnitine palmitoyltransferase I activity and promotion of fatty acid oxidation . G. cambogia extracts have potential as anti-obesity agents [19, 21, 22], and reduce the expression of the major adipogenic transcription factor, C/EBPα, in 3T3-L1 cells  and that of PPARγ, a nuclear hormone receptor involved in regulation of adipogenesis during differentiation .
l-Carnitine is an essential cofactor in the transport of long-chain fatty acids, such as acylcarnitine esters, across the inner mitochondrial membrane for subsequent fat degradation and energy production . l-Carnitine also functions in processes such as β-oxidation of long-chain fatty acids in peroxisomes, and the transfer of acetyl and other short-chain acyl groups from peroxisomes to mitochondria . Another role of l-carnitine is shuttling short-chain fatty acids from inside the mitochondria to the cytosol. Therefore, l-carnitine is responsible for maintaining the energy metabolism of the whole body . Expression of both PPARγ and adipose-specific fatty acid-binding protein (aP2), which are involved in adipogenesis, was found to be down-regulated by l-carnitine in 3T3-L1 adipocytes .
In the present study, the levels of leptin, TNF-α, and SREBP1c mRNA in epididymal adipose tissue were found to be decreased significantly in rats supplemented with the mixture. In conclusion, a mixture composed of G. cambogia aqueous extract, soy peptide and l-carnitine attenuated visceral fat accumulation and improved insulin resistance in a rat model with HFD-induced obesity, possibly through down-regulation of leptin, TNF-α, SREBP1c, and PPARγ2 gene expression in epididymal adipose tissue.
This work was supported by the Project of Bio-Food Research (# M10510130001-06N1013-00110) from the Korea Science and Engineering Foundation (KOSEF) under the Ministry of Science and Technology in Korea, and by the Brain Korea 21 Project, Yonsei University.
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