Exogenous Glucose Promotes Growth and Pectinase Activity of Bacillus licheniformis DY2 Through Frustrating the TCA Cycle
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Microbial pectinases are important sources due to the ease of production and unique physicochemical properties. Here, DY2, a strain of Bacillus licheniformis, was identified from 14 strains of bacteria as a pectinase-producing bacterium with good application potential. Optimized carbon sources of submerged fermentation led to the identification of glucose as an ideal carbon source for activity and production of P-DY2, the pectinase produced by DY2. GC-MS based metabolomics was used to explore metabolic mechanisms mediated by glucose, showing the frustrated TCA cycle is necessary to elevate the activity and production of P-DY2. Decreased activity of α-ketoglutaric dehydrogenase and succinate dehydrogenase of DY2 in glucose-treated samples supports the conclusion that P-DY2 production is the TCA cycle-independent. These results reveal a metabolic mechanism of high-activity pectinase mediated by exogenous glucose. These findings highlight the way to understand metabolic mechanisms and promote pectinase yield through metabolomics approach and metabolic modulation, respectively.
Keywordspectinase glucose TCA cycle enzyme activity metabolites
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This work was supported by National Natural Science Foundation of China (31701844), Natural Science Foundation of Fujian province (2018J05057) and Foundation of Fujian Educational Bureau (JAT170078).
- 4.Favela-Torres, E., T. Volke-Sepúlveda, and G. Viniegra-González (2006) Production of hydrolytic depolymerising pectinases. Food Technol. Biotechnol. 44: 221–227.Google Scholar
- 12.Utami, R., E. Widowati, A. Ivenaria, and E. Mahajoeno (2017) Polygalacturonase production by AR2 pectinolytic bacteria through submerged fermentation of raja nangka banana peel (Musa paradisiaca var. formatypica) with variation of carbon source and pectin. IOP Conf. Ser. Mater. Sci. Eng. 193: 012005.CrossRefGoogle Scholar
- 13.Bhardwaj, V. and N. Garg (2014) Pectinase production by Delftia acidovorans isolated from fruit waste under submerged fermentation. Int. J. Sci. Res. 3: 261–265.Google Scholar
- 28.Su, Y. B., B. Peng, H. Li, Z. Cheng, T. Zhang, J. Zhu, D. Li, M. Li, J. Ye, C. Du, S. Zhang, X. Zhao, M. Yang, and X. Peng (2018) Pyruvate cycle increases aminoglycoside efficacy and provides respiratory energy in bacteria. Proc. Natl. Acad. Sci. USA. 115: E1578–E1587.PubMedCrossRefPubMedCentralGoogle Scholar
- 30.Guan, Y., D. Yin, X. Du, and X. Ye (2018) Metabolomics approach used for understanding temperature-related pectinase activity in Bacillus licheniformis DY2. FEMS Microbiol. Lett. 365: fny255.Google Scholar
- 32.Zhou, C., J. Ye, Y. Xue, and Y. Ma (2015) Directed evolution and structural analysis of alkaline pectate lyase from the alkaliphilic bacterium Bacillus sp. strain N16-5 to improve its thermostability for efficient ramie degumming. Appl. Environ. Microbiol. 81: 5714–5723.PubMedPubMedCentralCrossRefGoogle Scholar
- 34.Joshi, M., M. Nerurkar, and R. Adivarekar (2013) Use of citrus limetta peels for pectinase production by marine bacillus subtilis. Innov. Rom. Food Biotechnol. 12: 75–83.Google Scholar
- 41.Gauthwal, M., D. Dahiya, and B. Battan (2015) Potential of a Bacillus aerius pectinase in fruit juice clarification produced by submerged fermentation using agri-residues. Int. J. Adv. Biotechnol. Res. 6: 394–400.Google Scholar
- 43.Guo, C., X. Y. Huang, M. J. Yang, S. Wang, S. T. Ren, H. Li, and X. X. Peng (2014) GC/MS-based metabolomics approach to identify biomarkers differentiating survivals from death in crucian carps infected by Edwardsiella tarda. Fish Shellfish Immunol. 39: 215–222.PubMedCrossRefPubMedCentralGoogle Scholar
- 45.Khakimov, B., L. D. Christiansen, A. L. Heins, K. M. Sorensen, C. Scholler, A. Clausen, T. Skov, K. V. Gernaey, and S. B. Engelsen (2017) Untargeted GC-MS metabolomics reveals changes in the metabolite dynamics of industrial scale batch fermentations of Streptoccoccus thermophilus broth. Biotechnol. J. 12: 170040.CrossRefGoogle Scholar