Biotechnology and Bioprocess Engineering

, Volume 23, Issue 6, pp 726–732 | Cite as

Production of Lipid Containing High Levels of Docosahexaenoic Acid by Cultivation of Aurantiochytrium sp. KRS101 Using Jerusalem Artichoke Extract

  • Jung-Hyun Ju
  • Baek-Rock Oh
  • Seung-Kyu Ryu
  • Sun-Yeon Heo
  • Su-Yeon Kim
  • Won-Kyung Hong
  • Chul Ho KimEmail author
  • Jeong-Woo SeoEmail author
Research Paper


In the present study, we evaluated extract of Jerusalem artichoke tubers (JAT) as a substrate for the production of lipid containing high levels of docosahexaenoic acid (DHA) by cultivated Aurantiochytrium sp. KRS101, an oleaginous protist. The optimal conditions for cultivation determined using response surface methods were as follows: pH, 5.9; enzyme loading, 282.6; and temperature, 27.7°C. The maximal levels of lipid (16.4 g/L) and productivity (3.6 g/L/d) were obtained during fed-batch fermentation, which achieved a DHA yield of 7.9 g/L (>48% of total fatty acids by weight). Moreover, JAT was more economical, reducing costs of expensive yeast extract as a nitrogen source by about 40%. These results suggest that JAT may be further explored as a low-cost feedstock for lipid production using microalgae strains.


oleaginous Aurantiochytrium lipid docosahexaenoic acid Jerusalem artichoke tubers 


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Copyright information

© The Korean Society for Biotechnology and Bioengineering and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Jung-Hyun Ju
    • 1
  • Baek-Rock Oh
    • 1
  • Seung-Kyu Ryu
    • 1
  • Sun-Yeon Heo
    • 1
  • Su-Yeon Kim
    • 1
    • 2
  • Won-Kyung Hong
    • 3
  • Chul Ho Kim
    • 1
  • Jeong-Woo Seo
    • 1
  1. 1.Industrial Microbiology & Bioprocess Research CenterJeonbuk Branch InstituteKRIBB, JeongeupKorea
  2. 2.Biosystems and Bioengineering KRIBB SchoolUniversity of Science and Technology (UST)DaejeonKorea
  3. 3.Korea Zoonosis Research Institute (KoZRI)Chonbuk National UniversityIksanKorea

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