Discovery of a RuBisCO-like Protein that Functions as an Oxygenase in the Novel d-Hamamelose Pathway
- 74 Downloads
Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) is the key catalyst of CO2 fixation in nature. RuBisCO forms I, II, and III catalyze CO2 fixation reactions, whereas form IV, also called the RuBisCO-like protein (RLP), is known to have no carboxylase or oxygenase activities. Here, we describe an RLP in Ochrobactrum anthropi ATCC 49188 (Oant_3067; HamA) that functions as an oxygenase in the metabolism of d-hamamelose, a branched-chain hexose found in most higher plants. The d-hamamelose pathway is comprised of five previously unknown enzymes: d-hamamelose dehydrogenase, d-hamamelono-lactonase, d-hamamelonate kinase, d-hamamelonate-2′,5-bisphosphate dehydrogenase (decarboxylating), and the RLP 3-keto-d-ribitol-1,5-bisphosphate (KRBP) oxygenase, which converts KRBP to 3-d-phosphoglycerate and phosphoglycolate. HamA represents the first RLP catalyzing the O2-dependent oxidative C–C bond cleavage reaction, and our findings may provide insights into its applications in oxidative cleavage of organic molecules.
KeywordsRuBisCO-like proteins oxygenase d-hamamelose metabolic pathway branched-chain sugar
Unable to display preview. Download preview PDF.
- 9.Bae, J., S. M. Kim, and S. B. Lee (2015) Identification and characterization of 2-keto-3-deoxy-L-rhamnonate dehydrogenase belonging to the MDR superfamily from the thermoacidophilic bacterium Sulfobacillus thermosulfidooxidans: implications to Lrhamnose metabolism in archaea. Extremophiles 19: 469–478.CrossRefGoogle Scholar
- 17.Hricovíniová, Z., M. Hricovíni, and L. Petruš (1998) Stereospecific molybdic acid-catalyzed isomerization of D-fructose to branched-chain aldose. The synthesis of D-hamamelose. Chem. Papers 52: 692–698.Google Scholar
- 18.Moore, S. and K. P. Link (1940) Carbohydrate Characterization I. The oxidation of aldoses by hypoiodite in methanol, II. The identification of seven aldo-monosaccharides as benzimidazole derivatives. J. Biol. Chem. 133: 293–311.Google Scholar
- 19.Weimberg, R. (1959) L-2-Keto-4,5-dihydroxyvaleric acid: an intermediate in the oxidation of L-arabinose by Pseudomonas saccharophila. J. Biol. Chem. 234: 727–732.Google Scholar
- 20.Wu, J. T., L. H. Wu, and J. A. Knight (1986) Stability of NADPH: effect of various factors on the kinetics of degradation. Clin. Chem. 32: 314–319.Google Scholar
- 27.Zhang, X., M. S. Carter, M. W. Vetting, B. San Francisco, S. Zhao, N. F. Al-Obaidi, J. O. Solbiati, J. J. Thiaville, V. de Crecy-Lagard, M. P. Jacobson, S. C. Almo, and J. A. Gerlt (2016) Assignment of function to a domain of unknown function: DUF1537 is a new kinase family in catabolic pathways for acid sugars. Proc. Natl. Acad. Sci. USA 113: E4161–4169.CrossRefGoogle Scholar
- 32.Portis, A. R. and M. A. Parry (2009) rubisco. In Encyclopedia of Life Sciences; John Wiley, Chichester, DOI: 10.1002/9780470015902.a0001293.pub2.Google Scholar