Biotechnology and Bioprocess Engineering

, Volume 23, Issue 5, pp 580–587 | Cite as

Intergeneric Hybridization between Streptomyces albulus and Bacillus subtilis Facilitates Production of ε-Poly-L-lysine from Corn Starch Residues

  • Shu Li
  • Nan Wang
  • Zong-Jun Du
  • Guan-Jun Chen
Research Paper


Intergeneric hybridization between S.albulus and B. subtilis to produce ε-poly-L-lysine (ε-PL) from corn starch residues (CSR) was investigated in this study. One hybrid, designated S. albulus LS-84, which incorporated the protease gene from B. subtilis, could effectively utilize the protein in CSR as a nitrogen source. In fed-batch fermentation, LS-84 produced 32.6 g/L ε-PL in the presence of 20 g/L CSR. This was an increase of 256.1% compared to that of the parent strain S. albulus LS-01. The rapid hydrolysis of CSR by protease caused rapid growth for LS-84, which allowed higher respiratory activity. As a result, activities of several key enzymes in LS-84 were higher than those in LS-01; additionally, the content of several intracellular amino acids, such as Asp, Glu, and Arg, was also much higher in LS-84. Therefore, intergeneric hybridization between S. albulus and B. subtilis to produce ε-PL from CSR is an economical method for effective utilization of waste resources.


ε-poly-L-lysine corn starch residues intergeneric hybridization 


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  1. 1.
    Shima, S. and H. Sakai (1977) Polylysine produced by Streptomyces. Agric. Biol. Chem. 41: 1807–1809.Google Scholar
  2. 2.
    Hiraki, J., T. Ichikawa, and S. Ninomiya (2003) Use of ADME studies to confirm the safety of polylysine as a preservative in food. Regul. Toxicol. Pharm. 37: 328–340.CrossRefGoogle Scholar
  3. 3.
    Kahar, P., T. Iwata, J. Hiraki, E. Y. Park, and M. Okabe (2001) Enhancement of e-polylysine production by Streptomyces albulus strain 410 using pH control. J. Biosci. Bioeng. 91: 190–194.CrossRefGoogle Scholar
  4. 4.
    Hirohara, H., M. Takehara, M. Saimura, A. Ikezaki, and M. Miyamoto (2006) Biosynthesis of poly(e-L-lysine)s in two newly isolated strains of Streptomyces sp. Appl. Microbiol Biotechnol. 73: 321–331.CrossRefGoogle Scholar
  5. 5.
    Chen, X. S., L. Tang, S. Li, L. J. Liao, J. H. Zhang, and Z. G. Mao (2011) Optimization of medium for enhancement of e-poly-Llysine production by Streptomyces sp. M-Z18 with glycerol as carbon source. Bioresour. Technol. 102: 1148–1159.Google Scholar
  6. 6.
    Xia, J., Z. X. Xu, H. Xu, J. F. Liang, S. Li, and X. H. Feng (2014) Economical production of poly(e-L-lysine) and poly(Ldiaminopropionic acid) using cane molasses and hydrolysate of streptomyces cells by Streptomyces albulus PD-1. Bioresour. Technol. 164: 241–247.CrossRefGoogle Scholar
  7. 7.
    Shima, S. and H. Sakai (1981) Poly-L-lysine produced by Streptomyces. Part II. Taxonomy and fermentation studies. Agric. Biol. Chem. 45: 2497–2502.Google Scholar
  8. 8.
    Wang, L., X. S. Chen, G. Y. Wu, X. Zeng, X. D. Ren, S. Li, and Z. G. Mao (2017) Enhanced e-poly-L-lysine production by inducing double antibiotic-resistant mutations in Streptomyces albulus. Bioprocess Biosyst. Eng. 40: 271–283.CrossRefGoogle Scholar
  9. 9.
    Li, S., L. Tang, X. S. Chen, L. J. Liao, F. Li, and Z. G. Mao (2011) Isolation and characterization of a novel e-poly-L-lysine producing strain: Streptomyces griseofuscus. J. Ind. Microbiol. Biotechnol. 38: 557–563.CrossRefGoogle Scholar
  10. 10.
    Li, S., X. S. Chen, C. D. Dong, F. L. Zhao, and Z. G. Mao (2013) Combining genome shuffling and interspecific hybridization among Streptomyces improved e-poly-L-lysine production. Appl. Biochem. Biotechnol. 169: 338–350.CrossRefGoogle Scholar
  11. 11.
    Hamano, Y. and I. Nicchu (2007) e-Poly-L-lysine producer, Streptomyces albulus, has feedback inhibition resistant aspartokinase. Appl. Microbiol. Biotechnol. 76: 873–882.CrossRefGoogle Scholar
  12. 12.
    Murmu, J. and C. William (2007) Phosphoenolpyruvate carboxylase protein kinase from developing castor oil seeds: partial purification, characterization, and reversible control by photosynthate supply. Planta 226: 1299–1310.CrossRefGoogle Scholar
  13. 13.
    Kiyohara, H., W. Toshiro, and I. Junko (1990) Intergeneric hybridization between Monascus anka and Asperyillus oryzae by protoplast fusion. Appl. Microbiol. Biotechnol. 33: 671–676.CrossRefGoogle Scholar
  14. 14.
    Rojan, P., D. Gangadharan, and K. Madhavan (2008) Genome shuffling of Lactobacillus delbrueckii mutant and Bacillus amyloliquefaciens through protoplasmic fusion for L-lactic acid production from starchy wastes. Bioresour. Technol. 99: 8008–8015.CrossRefGoogle Scholar
  15. 15.
    John, F. and E. Hendrik (1977) Interspecific hybridization between Penicillium chlysogenum and Penicillium cyaneofulvum following protoplast fusion. Mol. Gen. Genet. 157: 281–284.CrossRefGoogle Scholar
  16. 16.
    Takehara, M, and H. Hirohara (2010) in Amino-Acid Homopolymers Occurring in Nature. Springer-Verlag, Berlin, Germany, pp. 1–22.CrossRefGoogle Scholar
  17. 17.
    Driouch, H., B. Sommer, and C. Wittmann (2010) Morphology engineering of Aspergillus niger for improved enzyme production. Biotechnol. Bioeng. 105: 1058–1068.Google Scholar
  18. 18.
    Driouch, H., R. Hänsch, T. Wucherpfennig, R. Krull, and C. Wittmann (2012) Improved enzyme production by bio-pellets of Aspergillus niger: targeted morphology engineering using titanate microparticles. Biotechnol. Bioeng. 109: 462–471.CrossRefGoogle Scholar
  19. 19.
    Ren, X. D., Y. J. Xu, X. Zeng, X. S. Chen, L. Tang, and Z. G. Mao (2015) Microparticle enhanced production of e-poly- L-lysine in fed-batch fermentation. RSC Adv. 5: 82138–82143.CrossRefGoogle Scholar
  20. 20.
    Helena, B. and G. Hugh (1993) Phosphoenolpyruvate carboxylase from Streptomyces coelicolor A3(2): purification of the enzyme, cloning of the ppc gene and over-expression of the protein in a streptomycete. Biochem. J. 293: 131–136.CrossRefGoogle Scholar
  21. 21.
    Vrancken, G., T. Rimaux, and S. Weckx (2009) Environmental pH determines citrulline and ornithine release through the arginine deiminase pathway in Lactobacillus fermentum IMDO 13010. Int. J. Food Microbiol. 135: 216–222.CrossRefGoogle Scholar
  22. 22.
    Zhang, Y., J. Xu, and Z. Yuan (2010) Artificial neural networkgenetic algorithm based optimization for the immobilization of cellulase on the smart polymer eudragit L-100. Bioresour. Technol. 101: 3153–3158.CrossRefGoogle Scholar

Copyright information

© The Korean Society for Biotechnology and Bioengineering and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.College of Marine ScienceShandong University (Weihai)WeihaiChina
  2. 2.Weihai Food and Drug AdministrationTesting CenterWeihaiChina
  3. 3.College of Marine ScienceShandong University (Weihai)WeihaiChina

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