Biotechnology and Bioprocess Engineering

, Volume 23, Issue 5, pp 525–531 | Cite as

Cloning, Expression, and Characterization of a Thermotolerant β-agarase from Simiduia sp. SH-4

  • Jae-Deog Kim
  • Dong-Geun Lee
  • Sang-Hyeon LeeEmail author
Research Paper


The gene coding for a thermotolerant β-agarase from an isolated Simiduia sp. SH-4 was cloned, recombinantly expressed, and characterized after purification. This gene was sequenced after cassette mediated polymerase chain reaction and composed of an open reading frame of 1,809 base pairs, encoding a protein of 66.2 kilodaltons comprising of 602 amino acid residues. The amino acids sequence showed 74% homology with β-agarase of Simiduia agarivorans. A new β-agarase gene corresponding to mature protein of 577 amino acids was recombinantly expressed and purified by chitin bead column to homogeneity. The maximal specific activity was 505.07 U/mg at 50oC in Tris/HCl (pH 6.0) buffer. Recombinant β-agarase hydrolyzed agar into neoagarotetraose (57%) and neoagarohexaose (43%). It generated products from melted and non-melted powder agar and agarose at 30-50oC, meaning cheap agar materials could be used with energy- and costsavings. Thus, recombinant β-agarase could be used for industrial production of neoagarotetraose and neoagarohexaose.


β-agarase cloning expression purification Simiduia sp. 


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Copyright information

© The Korean Society for Biotechnology and Bioengineering and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Green-Chemistry Convergence Engineering, Graduate SchoolSilla UniversityBusanKorea
  2. 2.Department of Bioscience, Graduate SchoolSilla UniversityBusanKorea

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