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Biotechnology and Bioprocess Engineering

, Volume 22, Issue 4, pp 489–496 | Cite as

In vitro and in vivo investigation for biological activities of neoagarooligosaccharides prepared by hydrolyzing agar with β-agarase

  • Sun Joo Hong
  • Je-Hyeon Lee
  • Eun Joo Kim
  • Hea Jung Yang
  • Yong-Keun Chang
  • Jae-Seon Park
  • Soon-Kwang Hong
Research Paper

Abstract

Our study investigated the biological functions of three neoagarooligosaccharides (NAOs), neoagarobiose (NA2), neoagarotetraose (NA4), and neoagarohexaose (NA6). NAOs were prepared by hydrolyzing agar with the recombinant β-agarases, DagA and DagB, from Streptomyces coelicolor A3(2). An in vitro DPPH (diphenyl-2,4,6-trinitrophenyliminoazanium) radical scavenging assay revealed that none of the NAOs had any significant antioxidative activity. We also assessed the antibacterial activity of each NAO against several gram-negative and gram-positive bacteria. This revealed weak effects against four of the seven tested strains. NAO treatment resulted in significant inhibition of α-glucosidase, with the strongest effect observed in a NA4 and NA6 mixture, and a decreasing effect was observed in the order NA2>NA4>NA6. Little inhibition was observed against α-amylase, with an effect significantly lower than that of acarbose. No observable cytotoxicity was found in immortalized Melan-a mouse melanocytes by NAO treatment up to a concentration of 2,000 μg/mL. However, melanin synthesis was significantly reduced by NAO treatment, with the strongest effect observed in a NA4 and NA6 mixture and decreasing effects observed in the order NA6>NA4>NA2. The tyrosinase activity of Melan-a cells was also consistently decreased by NAO treatment. Among the tested factors, treatment of NA4 and NA6 mixture showed the highest inhibition effects against α-glucosidase and tyrosinase, and melanin biosynthesis. Further in vivo study to investigate the role of NAO in these biological functions will be necessary for future biotechnological applications.

Keywords

neoagarooligosaccharides α-glucosidase inhibition melanin biosynthesis tyrosinase inhibition antibacterial activity antioxidant activity 

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Copyright information

© The Korean Society for Biotechnology and Bioengineering and Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  • Sun Joo Hong
    • 1
    • 2
  • Je-Hyeon Lee
    • 2
  • Eun Joo Kim
    • 2
  • Hea Jung Yang
    • 2
  • Yong-Keun Chang
    • 3
  • Jae-Seon Park
    • 1
  • Soon-Kwang Hong
    • 1
  1. 1.Department of Biological Science and BioinformaticsMyongji UniversityYonginKorea
  2. 2.Dynebio Inc. B-B205 Woolimlions Valley IISeongnamKorea
  3. 3.Department of Chemical and Biomolecular EngineeringKorea Advanced Institute of Science and TechnologyTaejonKorea

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