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Biotechnology and Bioprocess Engineering

, Volume 21, Issue 6, pp 695–703 | Cite as

Improvement of 1,3-propanediol oxidoreductase (DhaT) stability against 3-hydroxypropionaldehyde by substitution of cysteine residues

  • Zhengbin Li
  • Soo Moon Ro
  • Balaji Sundara Sekar
  • Eunhee Seol
  • Suman Lama
  • Sun Gu Lee
  • Guangyi WangEmail author
  • Sunghoon ParkEmail author
Research Paper

Abstract

1,3-propanediol oxidoreductase (DhaT), which catalyzes the conversion of 3-hydroxypropionaldehyde (3-HPA) to 1,3-propanediol (1,3-PD) with the oxidation of NADH to NAD+, is a key enzyme in the production of 1,3-PD from glycerol. DhaT is known to be severely inactivated by its physiological substrate, 3-HPA, due to the reaction of 3-HPA with the thiol group of the cysteine residues. In this study, using site-directed mutagenesis, four cysteine residues in Klebsiella pneumoniae J2B DhaT were substituted to alanine, the amino acid commonly found in cysteine’s positions in other DhaT, individually and in combination. Among the total of 15 mutants developed, a double mutant (C28A_C107A) and a triple mutant (C28A_C93A_C107A) exhibited approximately 50 and 16% higher activity than the wild-type counterpart, respectively, after 1 h incubation with 10 mM 3-HPA. According to detailed kinetic studies, the double mutant had slightly better kinetic properties (V max , K cat , and K m for both 3-HPA and NADH) than wild-type DhaT. This study shows that DhaT stability against 3-HPA can be increased by cysteine-residue removal, albeit to a limited extent.

Keywords

1,3-propanediol oxidoreductase stability 3-hydroxypropionaldehyde cysteine site-directed mutagenesis 

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Copyright information

© The Korean Society for Biotechnology and Bioengineering and Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Zhengbin Li
    • 1
  • Soo Moon Ro
    • 2
  • Balaji Sundara Sekar
    • 3
  • Eunhee Seol
    • 3
  • Suman Lama
    • 3
  • Sun Gu Lee
    • 3
  • Guangyi Wang
    • 1
    Email author
  • Sunghoon Park
    • 3
    Email author
  1. 1.Center for Marine Environmental Ecology, School of Environmental Science and EngineeringTianjin UniversityTianjinChina
  2. 2.Interdisciplinary program of Clean TechnologyPusan National UniversityBusanKorea
  3. 3.Department of Chemical and Biomolecular EngineeringPusan National UniversityBusanKorea

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