Biotechnology and Bioprocess Engineering

, Volume 21, Issue 2, pp 294–298 | Cite as

Prohibitin deficiency causes opposing lipid metabolism between 3T3-L1 adipocytes and Clone 9 hepatocytes

Research Paper
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Abstract

Prohibitin (PHB) is a highly conserved protein in eukaryotic cells that are present in multiple cellular compartments and has potential roles as a tumor suppressor, an anti-proliferative protein, a regulator of cell-cycle progression and in apoptosis. In the present study, we generated PHB-deficient 3T3-L1 adipocytes and Clone 9 (C9) hepatocytes by oligonucleotide siRNA and investigated whether PHB affect lipid metabolism. It was revealed that PHB deficiency caused opposing lipid metabolism between the two cell models. PHB deficiency increased expression of adipogenic, lipogenic, and other lipid metabolic proteins in 3T3-L1 adipocytes, whereas significantly decreased the levels of those proteins in C9 cells. Collectively, PHB deficiency promoted lipid metabolism in 3T3-L1 adipocytes while it aggravated lipid metabolism in C9 hepatocytes.

Keywords

3T3-L1 Clone 9 prohibitin knockdown lipid metabolism 
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Copyright information

© The Korean Society for Biotechnology and Bioengineering and Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  1. 1.Department of BioindustryDaegu UniversityKyungsanKorea
  2. 2.Department of BiotechnologyDaegu UniversityKyungsanKorea

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